Aqueous Phytal extracts as source for staining in gel based protein separation techniques

  • Jayaprada Rao Chunduri Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
  • Harsha Mota Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
Keywords: PAGE, natural ingredients, staining solutions

Abstract

Gel based protein separation techniques such as PAGE is the best and easy methods among rapidly growing array of proteomic technologies. The post electrophoresis procedures usually comprise staining techniques to identify the separated proteins using CBB, Silver nitrate, fluorescent stains etc.  Chemical stains used in staining methods are non eco-friendly and non-degradable. Natural plant extracts used as stains for the development and identification of protein bands is a novel idea. Current study focuses on the development of new staining techniques using natural ingredients like tea, coffee, henna and beetroot etc which are easily available.  The results found to be 100% reliable, more ecofriendly, cost effective.  Natural staining procedures, their application in routine proteomic studies could be a step towards ecofriendly approaches in science.

Author Biographies

Jayaprada Rao Chunduri, Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
Asst Professor, Department of Biotechnology,Mithibai College
Harsha Mota, Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
Department of Biotechnology,Mithibai College

References

1. Gauci VJ, Wright EP and Coorssen J.R, Quantitative proteomics: assessing the spectrum of in-gel protein detection methods. J Chem Biol. 2011, 4(1), 3–29.
2. Chevalier F, Highlights on the capacities of "Gel-based" proteomics. Proteome Sci. 2010, 8-23.
3. Patton WF. Detection technologies in proteome analysis. J. Chromatography B 2002, 771: 3-31. Materials 2010, 3 4791
4. Diezel W, Kopperschläger G and Hofmann E, An improved procedure for protein staining in polyacryla-mide gels with a new type of Coomassie Brilliant Blue. Anal. Biochem. 1972, 48, 617-620.
5. Neuhoff V, Arold N, Taube D and Ehrhardt W. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 1988, 9, 255-262.
6. Mortz E, Krogh TN, Vorum H and Gorg A. Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics 2001, 1, 1359-1363.
7. Westermeier R, Marouga R. Protein detection methods in proteomics research. Bioscience Rep. 2005, 25, 19-32.
8. Berggren K, Chernokalskaya E, Steinberg TH, et al. Background-free, high sensitivity staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels using a luminescent ruthenium complex. Electrophoresis 2000, 21, 2509-2521.
9. Rabilloud T, Strub JM, Luche S, van Dorsselaer A and Lunardi J, Comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gels. Proteomics 2001, 1, 699-704.
10. Ali R, Sayeed SA, A plant dye from Lawsonia inermis for protein staining after polyacrylamide gel electro-phoresis. Electrophoresis, 1990, 11(4),343-344
11. Wadood A, Ghufran M, Jamal SB, Naeem M, Khan A, Phytochemical Analysis of Medicinal Plants Occur-ring in Local Area of Mardan, Biochem Anal Biochem 2013, 2, 144.
12. Tyagi R. K., Sagar R. and Datta K., A sensitive method for detection of proteins in polyacrylamide gels and on protein blots using acidic henna leaf extract, Biotechnology Techniques, 1994, 8(8), 583-588.
13. Chukwu OC, Odu C E, Chukwu D I, Hafiz N, Chidozie V N and Onyimba I A, Application of extracts of Henna (Lawsonia inamis) leaves as a counter stain, African Journal of Microbiology Research,2011, 5(21), 3351-3356.
14. Dong WH, Wang TY, Wang F, Zhang JH , Simple, Time-Saving Dye Staining of Proteins for Sodium Do-decyl Sulfate–Polyacrylamide Gel Electrophoresis Using Coomassie Blue. PLoS ONE,2011, 6(8): e22394.
15. Thakur N, Vikrant A, Preliminary Phytochemical Analysis of the Extracts of Psidium Leaves, Journal of Pharmacognosy and Phytochemistry,Middle-East Journal of Scientific Research,2014, 19 (11), 1421-1424.
16. François Chevalier, Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis, Materials 2010, 3, 4784-4792.
Published
2015-04-08
Section
Original Article