Activation Energy and Q10 values at various temperature ranges for alpha amylase activity in Telescopium telescopium
Keywords:
Activation energy, Telescopium telescopium, Q10 value, alpha amylase activity, hepatopancreas
Abstract
Enzyme alpha amylase was purified to the electrophoretic homogeneity from hepatopancreas of Telescopium telescopium by ammonium sulphate fractionation procedures, dialysis and chromatographic procedure. Purified enzyme had the activity equivalent to 42.12 µ mol/mg/min. Samples were collected from Choraon Islands of Goa State during 2004-2006. Energy of Activation was maximum between temperatures 10 0C - 20 0C ,187.38 ±10.43 J/mol; at 60 0C to 70 0C it was equivalent to about 154 J/mol. Q10 value was highest at a temperature range of 10 0C to 20 0C and the enzyme velocity elevated by a factor of 0.065 ± 0.009. As the temperature range was elevated beyond 20 0C up to 70 0C, the lowest Q10 value was obtained at a temperature range of 60 0C to 70 0C and it was equivalent to 0.001 ±0.00.References
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2. Joshi G. V. and B.B. Jamale(1975) ‘Ecological studies in mangroves of Terekhol and vashisti rivers’, p. 751-760.Bull Dept. Mar. Science University of Cochin-7, Volume-4.
3. A. E. Stearn. (1949) Kinetics of Biological Reactions, p. 25-41. In Advances in Enzymology and Related areas of Molecular Biology, Vol. 9. Edited by F. F.Nord.,Fordham Uni. N.Y. Interscience Publishers.
4. Benzinger, T. H. (1971), Thermodynamics, Chemical reactions and molecular biology. Nature (London). 229; 100-102.
5. Das S. Manna B. (1993) ‘Alimentary system of Telescopium telescopium’. Environment and Ecology’ Vol 11, p.283-291.
6. David T Plummer (1979). ‘An Introduction to Practical Biochemistry”;2ndedition, p.273-275. Mc Graw-Hill Publishing Co.Ltd., U.K.
7. Deutscher M.P.(1990), ‘Methods in Enzymology’ Vol. 182.(Guide to protein purification) Academic Press N.Y. p380-392.
8. Fagan J. M. (1986)’Demonstration of two distinct high molecular weight proteases in rabbit reticulocytes’.Jr. Biological Chemistry Vol. 262.No 6 pp 2451-2457.
9. Lowry OH, et al. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951:193:265-75.
Published
2016-04-27
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