2024-03-29T02:13:56Z
https://www.pacificejournals.com/journal/index.php/aabs/oai
oai:ojs.localhost:article/461
2015-12-28T11:06:40Z
aabs:L
Quality of Cold Centrifuge Affect on PCR Results
., Usha
Singh, Shailja
., Niladri
Rana, Ranjan Singh
Sharma, Kavyanjali
Kumar, Praveen
See Full text DNA extraction is an important step for PCR test. We are doing DNA extraction by salt-out and Phenol Chloroform method using two cold centrifuge instruments, one PSN Instrumentation Pvt. Ltd, Delhi Company and the other one Plasto Craft industries (P) Ltd. Mumbai. We performed HLAB-27 typing by conventional allele-specific PCR and the kit of BAG Company supplied by M/S Shiva Scientific. Using these two methods, we found that DNA extracted in PSN cold centrifuge gave false positive band in most cases while DNA extracted by Plasto Craft cold centrifuge did not give any false positive result in HLAB-27. Up till now it was considered that type and amount of sample, its collection, pipettting, quality of distilled water, contamination and quality of reagents, thermal cycle programme and gel electrophoresis affect PCR results. However our study suggest that quality of cold centrifuge also affect the quantity of DNA, PCR results and give false positive results and hence cold centrifuge should be of good quality and calibrated.
Pacific Group of e-Journals (PaGe)
2015-10-04
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs461
Annals of Applied Bio-Sciences; Vol 2 No 4 (2015); L1-2
2349-6991
2455-0396
eng
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs461/pdf_46
oai:ojs.localhost:article/881
2016-07-22T18:20:32Z
aabs:L
Mucopedermoid Carcinoma of Labial Mucosa
Tanveeer, Shahela
Kv, Karthik
P, Nishanth.
Afroz, Syed
Suri, Charu
P, Shravan
see full text
Pacific Group of e-Journals (PaGe)
2016-06-15
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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application/msword
application/msword
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs881
Annals of Applied Bio-Sciences; Vol 3 No 2 (2016); L1-4
2349-6991
2455-0396
eng
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs881/pdf_114
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs881/1058
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs881/1059
oai:ojs.localhost:article/1182
2017-01-02T19:42:13Z
aabs:L
Folic acid supplementation in pregnancy: Hitting many birds with one stone
Sharma, Ajitha
see full text
Pacific Group of e-Journals (PaGe)
2016-12-10
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs1182
Annals of Applied Bio-Sciences; Vol 3 No 4 (2016); L5
2349-6991
2455-0396
eng
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs1182/pdf_144
oai:ojs.localhost:article/1711
2017-12-24T02:07:23Z
aabs:L
Metanephric Adenoma
Malhotra, Aneeta Singh
Andotra, Urvashi
Rai, Harminder K
Khajuria, Arvind
Goswami, Kuldeep Chander
See full text DOI: 10.21276/AABS.1711
Pacific Group of e-Journals (PaGe)
2017-11-24
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
application/pdf
application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/vnd.openxmlformats-officedocument.wordprocessingml.document
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs1711
Annals of Applied Bio-Sciences; Vol 4 No 4 (2017); L1-2
2349-6991
2455-0396
eng
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs1711/pdf_202
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs1711/1384
https://www.pacificejournals.com/journal/index.php/aabs/article/view/aabs1711/1387