Aqueous Phytal extracts as source for staining in gel based protein separation techniques

  • Jayaprada Rao Chunduri Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
  • Harsha Mota Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
Keywords: PAGE, natural ingredients, staining solutions


Gel based protein separation techniques such as PAGE is the best and easy methods among rapidly growing array of proteomic technologies. The post electrophoresis procedures usually comprise staining techniques to identify the separated proteins using CBB, Silver nitrate, fluorescent stains etc.  Chemical stains used in staining methods are non eco-friendly and non-degradable. Natural plant extracts used as stains for the development and identification of protein bands is a novel idea. Current study focuses on the development of new staining techniques using natural ingredients like tea, coffee, henna and beetroot etc which are easily available.  The results found to be 100% reliable, more ecofriendly, cost effective.  Natural staining procedures, their application in routine proteomic studies could be a step towards ecofriendly approaches in science.

Author Biographies

Jayaprada Rao Chunduri, Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
Asst Professor, Department of Biotechnology,Mithibai College
Harsha Mota, Mithibai College, (affl to Mumbai University), Vile Parle(W), Mumbai
Department of Biotechnology,Mithibai College


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Original Article