Effect of temperature variation and Determination of optimum temperature of alpha amylase activity in Telescopium telescopium.
Keywords:
Amylase, Telescopium, Optimum Temperature
Abstract
Abstract The changes in enzyme activity of α- amylase from hepatopancreas of Telescopium telescopium from the Chorao Island of Goa State were investigated during year 2004 to 2006. The enzyme activity was observed highest at 20 minutes of incubation time and was 30.0 ± 10.83 µ mols/mg protein/min. The lowest enzyme activity was observed at zero minute of incubation and was 19.17 ± 1.23 µ mols/mg protein/min. At 40 0C of incubation temperature the highest enzymatic activity was observed and was 32.05 ± 3.307 µ mols/mg protein/min which is recorded as the optimum temperature. The enzyme activities were also noted for one week starved Telescopium telescopium in laboratory.References
References:
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1) Joshi G.V. and B.B. Jamale (1975)
In” Ecological studies in mangroves of Terekhol and Vashisti rivers” , p. 751-760. Bull Dept. Mar. Sci. University Cochin-7, vol-4.
2) Deutscher M.P.(1990) “Methods in enzymology” Vol.182.(Guide to protein purification) Academic Press New York. Pp 380- 392.
3) Fagan J.M. (1986), “Demonstration of two distinct high molecular weight proteases in rabbit reticulocytes”. Jr. Biological Chemistry Vol. 262.No 6,pp 2451- 2457.
4) Alexander P. and Block R.J.A. (1961).
In “Laboratory manual of Analytical methods of Protein Chemistry”, Vol.1-3, p.160- 161.: Pergamon Press,Oxford.
5) Lowry O. H. et al.(1951) “Protein measurement with the folin phenol reagent.”J.Biol. Chem. 193(1) :265-75.
6) Bertrand Tatoinkou fossi, Frederic tavea, (2004).
“Production and partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy soil”; African Jr. of Biotech. Vol 4(1), p.14-18.
7) Ching Yu Tsao,Yun-zu Pan, et al.(2003)
Purification and characterization of Amylases from small Abalone. Jr. Agric Food Chem. 51, p.1064- 1071.
8) Broekhuysen G. J.(1941) “A preliminary investigation of the importance of desiccation, temperature and salinity as factors controlling the vertical distribution of certain intertidal marine gastropods in False Bay, South Africa.”, Trans Roy Soc .S. Afr, 28,255
9) Evans R.G. (1948) “The lethal temperatures of some common british littoral molluscs” Jr.Anim.Ecology,17,165.
Published
2015-04-09
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