Mycological analysis of 150 cases of dermatophytosis of skin, hair and nail attending the outpatient department of skin and venereology

  • Bindu Mitruka Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
  • Amarjit Kaur Gill Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
  • Narinder Kaur Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
  • Ravinder K Mittal
  • Anchal Mahajan Adesh institute of medical sciences and research, Bathinda, punjab
  • Amandeep Kaur Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
Keywords: DERMATOPHYTOSIS, DIRECT MICROSCOPY, KOH PREPARATION, LCB MOUNT, SDA MEDIUM, TINEA RUBRUM, TINEA MENTAGROPHYTE

Abstract

BACKGROUND: Dermatophytes affect millions of people worldwide. Dermatophytosis inflicts a lot of psychosocial trauma. It is not generally appreciated how disabling a skin disease can be since an apparent trivial rash to the observer may be a source of intense discomfort and stigma to the patient.METHODS: The present study involved mycological analysis of 150 cases of dermatophytosis attending the OPD of Skin and Venereology, AIMSR, Bathinda during the period of 1st April 2014 to 30th September 2015. Detailed history was taken. Samples of skin, hair and nail were taken depending upon the part affected. Out of the material collected, part of it was used for direct KOH examination and remaining part was used to inoculate SDA medium with antibiotics for culture. Results of KOH preparation and culture, along with relevant history, were noted in Proforma. The observations and data obtained from the study were compiled and analyzed.RESULT: KOH examination was positive in 93 (62%) cases while culture was positive in 77 cases (51.34%) Overall, Trichophyton was the most common genus 76 samples (98.7%) isolated followed by Epidermophyton 1 sample. Out of the 77 culture positive cases, T. rubrum was the most common isolate in 51 cases (66.23%) followed by T. mentagrophytes in 22 cases (28.57%).                                                                                           CONCLUSION: It was concluded that KOH examination gives more positive results as compared to culture. Trichophyton infection is more common than Epidermophyton. T. rubrum is the most common infective dermatophyte out of all varieties. 

Author Biographies

Bindu Mitruka, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
Post Graduate StudentDepartment of Microbiology
Amarjit Kaur Gill, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
Professor and Head Department of Microbiology
Narinder Kaur, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
Associate ProfessorDepartment of Microbiology
Ravinder K Mittal
M.D,Skin & Venereal Diseases
Anchal Mahajan, Adesh institute of medical sciences and research, Bathinda, punjab
Assistant ProfessorDepartment of Microbiology
Amandeep Kaur, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab
Assistant ProfessorDepartment of Microbiology

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Published
2016-06-01
Issue
Vol 3 No 2 (2016)
Section
Original Article

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