A Study of Xylene Free Hematoxylin and Eosin Staining Procedure
Keywords:
Xylene, hematoxylin, detergents, occupational exposure, histological techniques
Abstract
Background: Mineral oils and liquid dish washing solutions have been tried by various investigators as a substitute for xylene in order to eliminate or minimize its toxic and hazardous effects. Methods: One hundred paraffin embedded sections each were stained by both conventional method and xylene free method using liquid dish washer. The sections were scored on the parameters of nuclear staining, cytoplasmic staining, crispness of staining, uniformity of staining and clarity of staining. The results of the two were compared and statistically analyzed.Result: The statistical analysis suggests that the soapy sections failed to prove that it has comparable effects as that of the conventional method.Conclusion: Liquid dish washer can be a safer non toxic alternative to xylene. More studies using modifications of the above technique can be undertaken to improve the quality of stained sections thus eliminating occupational hazards. DOI: 10.21276/AAMS.1772References
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procedure. J Orofac Sci 2012; 4:37-42
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hematoxylin and eosin staining. J Lab Physician 2014;6:84-90.
9. Udonkang M, Eluwa M, Ekanem T B, Asuquo OR, Akpantah AO. Bleached palm oil as substitute for xylene in histology. Journal of pharmacy and clinical sciences 2014; 8: 8-17.
10. Wikipedia contributors. Dishwashing liquid [Internet]. Wikipedia, The Free Encyclopedia; 2017 Sep 23, 12:47 UTC [cited 2017 Nov 22]. Available from:https://en.wikipedia.org/w/index.php?title=Dishwashing_liquid&oldid=802016653.
2. National Institute for Occupational Safety and Health (NIOSH) Occupational health guideline for xylene. Available from http://www.cdc.gov/niosh/docs/81- 123/pdfs/0668.pdf (Last accessed on 2015 Jan 26)
3. Rajan ST, Malathi N. Health hazards of xylene: a literature review. J Clin Diagn Res. 2014 ;8:271-4.
4. Kandyala R, Raghavendra SP, Rajasekharan ST. Xylene: An overview of its health hazards and preventive measures. J Oral Maxillofac Pathol 2010;14:1-5.
5. Buesa RJ, Peshkov MV. Histology without xylene. Ann Diagn Pathol 2009;13:246-56.
6. Ankle MR, Joshi PS. A study to evaluate efficacy of xylene free hematoxylin and eosin staining procedures as compared to the conventional hematoxylin and eosin staining. An experimental study. J Oral Maxillofac Pathol 2011; 15:161-7.
7. Ramulu S, Koneru A, Ravikumar S, Sharma P, Ramesh D, Patil R. Liquid dish washing soap : An excellent substitute for xylene and alcohol in hematoxylin and eosin staining
procedure. J Orofac Sci 2012; 4:37-42
8. Pandey P, Dixit A, Tanwar A, Sharma A, Mittal S. A comparative study to evaluate liquid dish washing soap as an alternative to xylene and alcohol in deparaffinization and
hematoxylin and eosin staining. J Lab Physician 2014;6:84-90.
9. Udonkang M, Eluwa M, Ekanem T B, Asuquo OR, Akpantah AO. Bleached palm oil as substitute for xylene in histology. Journal of pharmacy and clinical sciences 2014; 8: 8-17.
10. Wikipedia contributors. Dishwashing liquid [Internet]. Wikipedia, The Free Encyclopedia; 2017 Sep 23, 12:47 UTC [cited 2017 Nov 22]. Available from:https://en.wikipedia.org/w/index.php?title=Dishwashing_liquid&oldid=802016653.
Published
2017-12-09
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Section
Original Article
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