Interaction of Human Dental Pulp Stem Cells and Ameloblastic Cell In-vitro- A Preclinical Analysis

  • K Manimaran Department Of Oromaxillofacial Surgery, KSR Institute of Dental Science and Research. Tiruchengode, TN, India
  • Arun Kumar
  • Avinash Gandi D Bharathidasan University
  • S Sankaranarayanan
Keywords: Human Dental Pulp Stem Cells, Ameloblastic Cell, in-vitro


Background: To report in-vitro interaction between ameloblastic cell and dental pulp stem cell.Methods: Dental Pulp stem cells were isolated from extracted pre molars and Ameloblastoma primary culture was obtained from the patient. Direct co-culture study was performed to do morphological sorting with tryphan blue dye exclusion method and transwell co-culture was performed to study anti-proliferative effects.Results: The viability count from transwell co-culture plates revealed that in the control wells the AM has higher proliferation rate than DPSC. However when co-cultured with DPSCs, the proliferation level is arrested and decreased cell count was noted. This was confirmed by Tryphan blue dye exclusion assay also.Conclusion: Result demonstrated that dental pulp stem cell can induce anti-proliferation or anti-tumorigenic activity in ameloblastoma cells. This has helped us in using these cells in the management of ameloblastoma cases.

Author Biography

Avinash Gandi D, Bharathidasan University
Scientific Co ordinatorDept. of Regenerative medicine


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Original Articles