Interaction of Human Dental Pulp Stem Cells and Ameloblastic Cell In-vitro- A Preclinical Analysis
Keywords:
Human Dental Pulp Stem Cells, Ameloblastic Cell, in-vitro
Abstract
Background: To report in-vitro interaction between ameloblastic cell and dental pulp stem cell.Methods: Dental Pulp stem cells were isolated from extracted pre molars and Ameloblastoma primary culture was obtained from the patient. Direct co-culture study was performed to do morphological sorting with tryphan blue dye exclusion method and transwell co-culture was performed to study anti-proliferative effects.Results: The viability count from transwell co-culture plates revealed that in the control wells the AM has higher proliferation rate than DPSC. However when co-cultured with DPSCs, the proliferation level is arrested and decreased cell count was noted. This was confirmed by Tryphan blue dye exclusion assay also.Conclusion: Result demonstrated that dental pulp stem cell can induce anti-proliferation or anti-tumorigenic activity in ameloblastoma cells. This has helped us in using these cells in the management of ameloblastoma cases.References
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2. Qiao L, Xu Z, Zhao T, Zhao Z, Shi M, Zhao RC, et al. Suppression of tumorigenesis by human mesenchymal stem cells in a hepatoma model. Cell Res. 2008;18:500-7.
3. Khakoo AY, Pati S, Anderson SA, Reid W, Elshal MF, Rovira II, et al. Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma.IntJExp Med. 2006;203:1235-47.
4. Perry B C, Zhou D, Wu X et al. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. Tissue Engineering Part C Methods 2008;14:149-156.
5. Stenman G, Lilja J, and Sagne S. Human ameloblastomas in vitro: Light microscopical and ultrastructural observations.Br J Oral Maxillofac Surg.1985;23: 326-32.
6. Darlington G J. Viability Staining of Mammalian Cell Cultures. CSH Protoc 2007;2007: pdb.prot4769
7. Yamaza T, Kentaro A, Chen C et al. Immunomodulatory properties of stem cells from human exfoliated deciduous teeth. Stem cell research & therapy 2010;1:5.
8. Kern S, Eichler H, Stoeve J et al. Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 2006;24:1294-1301.
9. Morsczeck C, Schmalz G, Reichert TE et al. Somatic stem cells for regenerative dentistry. Clin Oral Investig2008;12:113-118.
10. Nauta A J and Fibbe WE. Immunomodulatory properties of mesenchymal stromal cells. Blood 2007;110: 3499-3506.
11. Uccelli A, Moretta L, and Pistoia V. Mesenchymal stem cells in health and disease. Nature Reviews Immunology 2008;8:726-736.
- In-vitro culture of Dental Pulp Stem Cells (DPSC) and Ameloblastoma (AM) cells
- Tryphan Blue staining of culture of Dental Pulp Stem Cells (DPSC) and Ameloblastoma (AM) cells demonstrating viable DPSC and stained (dead) AM cells (A) before staining (B) after staining (Arrows) (Magnification x200).
- Tryphan blue viability staining showed Proliferation of Dental Pulp Stem Cells (DPSC) and Ameloblastoma (AM) cells in the control wells but in the co culture trans wells Proliferation of DPSC alone was noted and the AM cells showed decreased population as
Published
2018-03-24
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