Special stains in study of infertile endometrium
Keywords:
Endometrium, Infertility, Special stains
Abstract
Background: Integrity of ovarian function is a prerequisite for successful implantation of ovum. The subtle changes in ovarian function can be detected by studying endometrial carbohydrates with special stains.Material and methods: 87 infertile and 30 normal endometria were studied with the help of Hematoxylin& Eosin ( H & E) stain and special stains like Best Carmine, Alcian blue PAS (with and without diastase digestion) and reticulin stain.Results: Special stains were useful in emphasizing the cyclical activity of carbohydrates and stroma in normal endometrium. In infertile endometrium, they helped in precise classification and detection of corpus luteal deficiency, at times (8.7% cases) missed on routine H& E stain . Intrinsic abnormalities like polyps and tuberculous granulomas could be detected with ease on reticulin stain. On application of special stains, incidence of corpus luteal deficiency was 27.5%, and that of anovulatory infertility and normal secretory endometrium was 34.5% each. Conclusion: The special stains helped in detecting precise endometrial response and added to the information obtained by routine H&E stain.DOI:10.21276/APALM.1725References
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10. Salma M, Kouser T, Nasar MA. Endometrial Hyperplasia: a 5 - Years Retrospective Study. IJPO. 2016; 3(2): 221-25.
2. Dallenbach- Hellweg G. The endometrium of infertility. A review. Pathol Res Pract. 1984 ; 178(6):527-37.
3. Spirtos NJ, Yurewicz EC, Moghissi KS, Magyar DM, Sundareson AS, Bottoms SF. Pseudocorpus luteum insufficiency: A study of cytosol progesterone receptors in human endometrium. Obstet Gynecol 1985; 65: 535-40.
4. Zawar MP, Deshpande NM, Gadgil PA, Mahanta AA. Histopathological study of endometrium in infertiity. Indian J Pathol Microbiol. 2003; 46: 630-3.
5. Sahasrabudhe NS, Shinde S, Jadhav MV. Endometrium in infertility. Indian Journal of Obstetrics and Gynaecology. 2001; 51: 100-2.
6. Meayama M, Ikuzo S, Matsuol I, Nakahara K. Glycogen estimation by rapid enzymatic method in human endometrium. Glycogen content in the endometrium of infertile patients during the menstrual cycle. Fertil Steril.1977; 28: 159- 2.
7. Schoon HA, Wiegandt I, Schoon D, Aupperle H, Bartmann CP. Functional disturbances in the endometrium of barren mares: a histological and immunohitological study. J Reprod Fertil Suppl. 2000; 56: 381-91.
8. Hoffmann C,Bazer FW, Klug J, Aupperle H, Ellenberger C, Schoon HA. Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium Expression patterns for mares suffering from endometrosis. Theriogenology. 2009;71(2):264-74.
9. Devi LS, Singh MR, Singh LR, Debnath K. The histological and histochemical study of endometrium in dysfunctional uterine bleeding. J Med Soc. 2012; 26: 167-70.
10. Salma M, Kouser T, Nasar MA. Endometrial Hyperplasia: a 5 - Years Retrospective Study. IJPO. 2016; 3(2): 221-25.
Published
2018-04-28
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