Comparative Study of Platelet Indices in Normal Pregnant and Non-Pregnant Women in a Tertiary Care Hospital in Northern India
Keywords:
Platelets, platelet indices, pregnancy, normal, trimester, automated
Abstract
Aims and Objectives: To compare Platelet Indices between pregnant females and non-pregnant females attending the Antenatal Clinic in a tertiary care hospital. Platelet Indices amongst pregnant women between the three trimesters were also compared. Materials and Methods: A case control study was conducted in the Department of Pathology, ESIC Medical College and Hospital, Faridabad, Haryana India on 119 normal pregnant females and 119 non-pregnant normal females in the reproductive age group (18 to 45 years) with the approval of the ethical committee. Venous blood was collected from all participants in EDTA vacutainer and CBC was performed using fully automated analyser, XN 1000 within 2 hours of sample collection. The data were analysed using SPSS software 17.0. Unpaired Student t-test was applied to compare the various platelet indices between the cases and controls. One-way ANOVA was applied to study between group differences among the three different trimesters of pregnancy for the platelet counts and indices. Results: Platelet count was found to be higher among controls as compared to cases and this was found to be statistically significant (p=0.017). Platelet count was highest among subjects in the first trimester and subsequently decreased among subjects in the second and third trimester. But was not statistically significant. An inverse relationship was seen between MPV and Platelet Count, this was statistically significant. Conclusion: The Platelet count and Indices were reduced in normal pregnancy. However, owing to a small sample size, the statistical significance of various platelet indices between cases and controls and among the three trimesters were limited.References
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2 Hoak JC. Platelet and atherosclerosis. Semin Thromb Hemost 1988;14:202–205.
3 Golebieswska EM, Poole AW. Platelet secretion: From hemostasis to wound healing and beyond. Blood Rev 2015;29:153-62 .
4 Lopez E, Bermejo N, Berna-Erro A, Alonso N, Salido GM, Redondo PC et al. Relationship between calcium mobilization and platelet α- and δ-granule secretion. A role for TRPC6 in thrombin-evoked δ-granule exocytosis. Arch Biochem Biophys 2015;585:75-81.
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7 Abass AE, Abdalla R. Evaluation of platelet count and indices in pre- eclampsia compared to normal pregnancies. Journal of Dental and Medical Sciences 2016 July;15(7):05 -08.
8 Goshal MK, Bhattecharyya M. Overview of platelet physiology: its hemostatic and nonhemostatic role in disease pathogenesis. The Scientific World Journal 2014:1-16.
9 Dundar O, Yoruk P, Tutuncu L. Longitudinal study of platelet size changes in gestation and predictive power of elevated MPV in development of preeclampsia. Prenat Diag 2008;28:1052-6.
10 Sheu JR, Hsiao G, Shen MY, Lin WY, Tzeng CR. The hyperaggregability of platelets from normal pregnancy is mediated through thromboxane A2 and cyclic AMP pathways. Clin Laboratory Haematol 2002;24:121–9.
11 Fay RA, Hughes AO, Farron NT. Platelets in pregnancy: hyperdestruction in pregnancy. Obstet Gynecol 1983;61:238-40.
12 Thachil J. Platelets in inflammatory disorders: a pathophysiological and clinical perspective. Semin Thromb Hemost 2015;41:572-81.
13 Margetic S. Inflammation and haemostasis. Biochem Med (Zagreb) 2012;22:49–62.
14 Jaremo TL, Lindahl C, Forsgren H. The use of platelet density and volume measurements to estimate the severity of pre-eclampsia. Eur J Clin Inv 2000;30:1113–8.
15 Larsen SB, Grove EL, Hvas AM, Kristensen SD. Platelet turnover in stable coronary artery disease-influence of thrombopoietin and low-grade inflammation. PLoS One 2014;9:e85566.
16 Brown AS, Hong Y, De AB, Beacon H, Beeso J, Sherwood R et al. Megakaryocyte ploidy and platelet changes in human diabetes and atherosclerosis. Arterioscler Thromb Vasc Biol 1997;17:802–7.
17 Osselaer JC, Jamart J, Scheiff JM. Platelet distribution width for differential diagnosis of thrombocytosis. Clin Chem 1997;43:1072–6.
18 Mariani E, Filardo G, Canella V, Berlingeri A, Bielli A, Cattini L et al. Platelet-rich plasma affects bacterial growth in vitro. Cytotherapy 2014;16:1294-304.
19 Yang A, Pizzulli L, Luderitz B. Mean platelet volume as marker of restenosis after percutaneous transluminal coronary angioplasty in patients with stable and unstable angina pectoris. Thromb Res 2006;117:371–7.
20 Chandrashekar V. Plateletcrit as a screening tool for detection of platelet quantitative disorders. J Hematol 2013;2:22– 6.
21 Giacomini A, Legovini P, Gessoni G, Antico F, Valverde S, Salvadego MM et al. Platelet count and parameters determined by the Bayer ADVIA 120 in reference subjects and patients. Clin Lab Haematol 2001;23:181–6.
22 Adibi P, Imani EF, Talaei M, Ghanei M. Population-based platelet reference values for an Iranian population. Int J Lab Hematol 2007;29:195-9.
23 Vagdatli E, Gounari E, Lazaridou E, Katsibourlia E, Tsikopoulou F, Labrianou I. Platelet distribution width: a simple, practical and specific marker of activation of coagulation. Hippokratia 2010;14:28-32.
24 Babu E, Basu D. Platelet large cell ratio in the differential diagnosis of abnormal platelet counts. Indian J Pathol Microbiol 2005 Jan;48(1):35.
25 Hong H, Xiao W, Maitta RW. Steady increment of immature platelet fraction is suppressed by irradiation in single-donor platelet components during storage. PLoS One 2014;9:e85465.
26 Pughikomo OC, Pughikomo DT, Iyalla C. Platelet indices in pregnant women in Port Harcourt, Nigeria. Journal of Dental and Medical Sciences 2015;14 (3): 28-31.
2 Hoak JC. Platelet and atherosclerosis. Semin Thromb Hemost 1988;14:202–205.
3 Golebieswska EM, Poole AW. Platelet secretion: From hemostasis to wound healing and beyond. Blood Rev 2015;29:153-62 .
4 Lopez E, Bermejo N, Berna-Erro A, Alonso N, Salido GM, Redondo PC et al. Relationship between calcium mobilization and platelet α- and δ-granule secretion. A role for TRPC6 in thrombin-evoked δ-granule exocytosis. Arch Biochem Biophys 2015;585:75-81.
5 Juan P, Stefano G, Antonella S, Albana C. Platelets in Pregnancy. J Prena Med 2011 Oct – Dec;5(4):90-2.
6 Valera M, Parant O, Vayssiere C, Arnal JO, Payrastre B. Physiologic. Pathologic changes of platelets in pregnancy. Platelets 2010;21(8):587-95.
7 Abass AE, Abdalla R. Evaluation of platelet count and indices in pre- eclampsia compared to normal pregnancies. Journal of Dental and Medical Sciences 2016 July;15(7):05 -08.
8 Goshal MK, Bhattecharyya M. Overview of platelet physiology: its hemostatic and nonhemostatic role in disease pathogenesis. The Scientific World Journal 2014:1-16.
9 Dundar O, Yoruk P, Tutuncu L. Longitudinal study of platelet size changes in gestation and predictive power of elevated MPV in development of preeclampsia. Prenat Diag 2008;28:1052-6.
10 Sheu JR, Hsiao G, Shen MY, Lin WY, Tzeng CR. The hyperaggregability of platelets from normal pregnancy is mediated through thromboxane A2 and cyclic AMP pathways. Clin Laboratory Haematol 2002;24:121–9.
11 Fay RA, Hughes AO, Farron NT. Platelets in pregnancy: hyperdestruction in pregnancy. Obstet Gynecol 1983;61:238-40.
12 Thachil J. Platelets in inflammatory disorders: a pathophysiological and clinical perspective. Semin Thromb Hemost 2015;41:572-81.
13 Margetic S. Inflammation and haemostasis. Biochem Med (Zagreb) 2012;22:49–62.
14 Jaremo TL, Lindahl C, Forsgren H. The use of platelet density and volume measurements to estimate the severity of pre-eclampsia. Eur J Clin Inv 2000;30:1113–8.
15 Larsen SB, Grove EL, Hvas AM, Kristensen SD. Platelet turnover in stable coronary artery disease-influence of thrombopoietin and low-grade inflammation. PLoS One 2014;9:e85566.
16 Brown AS, Hong Y, De AB, Beacon H, Beeso J, Sherwood R et al. Megakaryocyte ploidy and platelet changes in human diabetes and atherosclerosis. Arterioscler Thromb Vasc Biol 1997;17:802–7.
17 Osselaer JC, Jamart J, Scheiff JM. Platelet distribution width for differential diagnosis of thrombocytosis. Clin Chem 1997;43:1072–6.
18 Mariani E, Filardo G, Canella V, Berlingeri A, Bielli A, Cattini L et al. Platelet-rich plasma affects bacterial growth in vitro. Cytotherapy 2014;16:1294-304.
19 Yang A, Pizzulli L, Luderitz B. Mean platelet volume as marker of restenosis after percutaneous transluminal coronary angioplasty in patients with stable and unstable angina pectoris. Thromb Res 2006;117:371–7.
20 Chandrashekar V. Plateletcrit as a screening tool for detection of platelet quantitative disorders. J Hematol 2013;2:22– 6.
21 Giacomini A, Legovini P, Gessoni G, Antico F, Valverde S, Salvadego MM et al. Platelet count and parameters determined by the Bayer ADVIA 120 in reference subjects and patients. Clin Lab Haematol 2001;23:181–6.
22 Adibi P, Imani EF, Talaei M, Ghanei M. Population-based platelet reference values for an Iranian population. Int J Lab Hematol 2007;29:195-9.
23 Vagdatli E, Gounari E, Lazaridou E, Katsibourlia E, Tsikopoulou F, Labrianou I. Platelet distribution width: a simple, practical and specific marker of activation of coagulation. Hippokratia 2010;14:28-32.
24 Babu E, Basu D. Platelet large cell ratio in the differential diagnosis of abnormal platelet counts. Indian J Pathol Microbiol 2005 Jan;48(1):35.
25 Hong H, Xiao W, Maitta RW. Steady increment of immature platelet fraction is suppressed by irradiation in single-donor platelet components during storage. PLoS One 2014;9:e85465.
26 Pughikomo OC, Pughikomo DT, Iyalla C. Platelet indices in pregnant women in Port Harcourt, Nigeria. Journal of Dental and Medical Sciences 2015;14 (3): 28-31.
Published
2018-08-23
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