Bacteriological Study of Wound Infections and Their Antibiogram Pattern at A Tertiary Care Hospital in Suburban Area of Hyderabad
Keywords:
Staphylococcus aureus, Pseudomonas aeruginosa, monobacterial
Abstract
Background: Wound infections can be caused through two major sources: exogenous and endogenous bacteria. The probability of wound infection largely depends on the patient’s systemic host defenses, local wound conditions and microbial burden. Despite modern surgical techniques and the use of antibiotic prophylaxis, Surgical Site Infection (SSI) is one of the most common complications encountered in surgery. SSI places a significant burden on both the patient and health system. SSI is thus a major cause of morbidity, prolonged hospital stays and increased health costs. Methods: Two wound swabs were collected from the wound and from a drop of aspirate, smear was made on clean glass slide and Gram staining was done for direct microscopic examination under oil immersion 100X objective to know various morphological types of bacteria and presence or absence of inflammatory cells. Second swab/drop of aspirate was used for culture by inoculating it on routine media like Blood Agar, Nutrient Agar and MacConkey’s agar, incubated at 37° C for 24 hrs. aerobically. Result: Out of 108 pus samples, 101(88.5%) were culture positive for bacterial growth and no growth was observed in 7(11.4%) cases. Out of 101 bacterial culture positive cases, 96 were nonbacterial and 5 were poly bacterial. Out of 96 bacterial isolates; S. aureus (38/36.6%) was the commonest followed by P. aeruginosa (26/24.7%) Conclusion: The study concludes that variety of aerobic bacteria is responsible for wound infection with predominance of Staphylococcus aureus followed by Pseudomonas aeruginosa.References
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[2]. Patricia Tille Text book of Diagnostic Microbiology, Bailey and Scott’s. Thirteenth Edition Pg 961-970.
[3]. Lauwers S, F De Smet, Surgical Site Infections Acta Clinica Belgica 1998, 53-5; 303-310.
[4]. Culbertson WR, Altemeier WA, Gonzalez LC, Hill EO. Studies on the epidemiology of the post – operative infection of clean operative wounds, Ann Surg 1961, 154(4):599-603.
[5]. M Olson, MO’ Connor, ML Schwartz. Surgical wound infections. of 5 year prospective study of 20, 193 wounds at Minneapolis Va Medical Centre. Ann Surg, 1984 Mar; 199(3): 253-259.
[6]. Dellinger, E.P in Sabiston, D.C, Lyerly, K. (Eds) Textbook of Surgery, 18th Edn. WB Saunders Company, Philladilphia, PP-264-280.
[7]. Forber BA, Salm DF, Weissfiled Diagnostic Microbiology. In Bailey and Scott’s, 13th edition; Missiouri, Mosby Elsevier, 1998.
[8]. Washington CW, Stephen DA, William M, Elmer WK et al. In Koneman’s Colour atlas and text book of microbiology. 6th edition; Philadelphia; Lippincotts Williams and Walkins’ 2006; 68-74.
[9]. Clinical and Laboratory Standard Institute, Performance Standards for antimicrobial susceptibility testing, twenty third informational supplement, M100-S24, January 2017 Vol 34, No.P:50-57.
[10]. Miles RS, Anyes SGB. Laboratory Control of antimicrobial therapy, in General Colle J, Barrie P, Andrew GF, Anthony S, editors. Mackie and Mc Cartney Practical Medical Microbiology; 14th edition; New Delhi; 2007, 151-178.
[11]. Malik S, Gupta A, Singh KP, Agarwal J, Singh M. Antibiogram of Aerobic Bacterial Isolates from Post-Operative wound Infections at a Tertiary Care Hospital in India. J. Infect. Dis-Antimicriob Agents. Jan-Apr-2011; 28; 45-51.
[12]. Afroz Z, Metri BC, Jyoti P. Bacteriological Profile and Antimicrobial Susceptibility Pattern of Skin and Soft Tissue Infections among Gram Negative Bacilli in a Tertiary Care Hospital of South India. J. Pharm. Scie & Res. 2015, 7(7); 397-400.
[13]. Rao R, Sumathi S, Anuradha K, Venkatesh D, Krishna S. Bacteriology of Post Operative wound infections .Int. J. Pharm Biomed Res 2013, 4(2), 72-76.
[14]. Sowmya N, Savitha S, Mallive S, Mohanakrishnan K, Sumathi G, Arumugam P. A two year study of spectrum of bacterial isolates from wound infections by aerobic culture and their antibiotic pattern in a tertiary care centre. Int. J. Cure. Microbiol, App. Sci (2014) 3(8) 292-295.
[15] Mehta VJ, Pandya JM, Mehta SJ, Surveillance of Post Operative wound infections in a Teaching Hospital, Gujarat. Int J Res Med. 2013; 2(4); 77-79.
[16]. Naik G, Srinivas R, Desphande. A Study on Surgical Site Infections caused by Staphylococcus aureus with a special search of Methicillin- Resistant isolates. Journal of Clinical and Diagnostic Research, 2011;5(3): 502-508.
[17]. Wanshi PS, Khan AQ, Ali S, Patil A. Analysis of Organisms Found at Incision site Intra-Operatively and its Implications with Post-Operative Infections. Int. J. of Sci and Res. Publications, 2014 Vol 4(4)
[18]. Anbumani N, Kalyani J, Mallika M. Prevalence of methicillin resistant Staphylococcus aureus in a tertiary referral hospital in Chennai, South India. Ind J Pract Doc 2006, 48 (4); 191-195.
[19]. Rajaduraipandi K, Mani KR, Pannerselvam K, M Mani, M Bhaskar, P Manikandan. Prevalence and antimicrobial pattern of methicillin resistant Staphylococcus aureus: A multicentre study. Ind J. Med. Micro 2006, 24(1): 34-38.
[20]. Anupurba S, Sen MR, Nath G, Sharma BM, Gulati and Mohapatra TM. Prevalence of Methicillin Resistant Staphylococcus aureus in a Tertiary Referral Hospital in Uttar Pradesh, India J. of Medical microbiology 2003; 21 :49-51.
[21]. Shriyan A, Sheetal R, Nayak N .Aerobic micro-organisms in Post-Operative wound infections and their Antimicrobial suspceptibility Patterns. Journal of Clinical and Diagnostic Research 2010 December; 4; 3392-3396.
[22]. Javed FS, Reazvanian M, Kumar S, Norouzi H. Microbiological study of diabetic foot ulcer in an Indian Tertiary Care Hospital. Int Journal of Life Sci and Review 2015:1(2); 65-70.
[23]. Amatya J, Rijal M, Baidya R. Bacteriological study of the Post Operative wound samples and antibiotic susceptibility pattern of the isolates in B&B Hospital Lalitpur Nepal. JSM Microbiology 2015, 3(1): 1019.
[24]. Rai S, Yadav U N, Pant N D, Yakha J K, Tripathi PP, Poudel A, Lekhak B. Bacteriological Profile and Antimicrobial Susceptibility Patterns of Bacteria Isolated from Pus/Wound Swab Samples from Children Attending a Tertiary Care Hospital in Kathmandu, Nepal. International journal of Microbiology 2017: 1(6): 54-59
Published
2018-09-17
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