Effects of Site of Blood Collection and Duration of Storage on Coagulation Factor V and Factor IX Levels in Fresh Frozen Plasma

  • Loganathan R Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India
  • Rajendra G Kulkarni Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India
  • Rakhee Kar Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India
  • B Abhishekh Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India
  • Debdatta Basu Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India
DOI: https://doi.org/10.21276/APALM.2822
Keywords: Factor V, Factor IX, Blood bank and camp site, Fresh Frozen plasma

Abstract

Background: As per Indian drugs and cosmetic act & rules 1945 (DCA), 1% or 4 units/month of all components should meet quality control parameter. Aims and objectives: 1.) To assess the level of clotting factors FV and FIX in FFP. 2.) To identify the association between clotting factors FV and FIX in FFP with its mode of collection and storage. Methods: This is a Cross sectional study design done for a period of 20 months. The comparison of the levels of clotting factors such as V and IX between the blood group were carried out by using independent students’ one-way analysis of variance. All statistical analysis was carried out at 5% level of significance and p-value <0.05 were considered as significant. All statistical analysis was done using software IBM PASW statistics (SPSS) version 19.0. Result: The present study was done at a south Indian tertiary care center from January 2016 to August 2017. Around 28,919 FFPs were assessed during this study period. On comparing factor levels between blood bank and camp site, there were no significant difference. Conclusion: In our study, FV and FIX were maintained as per DGHS criteria.

References

1. Fasano R, Luban NLC. Blood Component Therapy. Pediatr Clin North Am. 2008 Apr;55:421–45.
2. Chng WJ, Tan MK, Kuperan P, Wee Joo C. An Audit of Fresh Frozen Plasma Usage in An Acute General Hospital in Singapore. Singapore Med J. 2003;44:574–8.
3. Saran R. Transfusion medicine Technical Manual. 2nd ed. New Delhi: Directorate General of Health Services; 2003.
4. Arya RC, Wander G, Gupta P. Blood component therapy: Which, when and how much. J Anaesthesiol Clin Pharmacol. 2011 Apr;27:278–84.
5. Agus N, Yilmaz N, Colak A, Liv F. Levels of factor VIII and factor IX in fresh-frozen plasma produced from whole blood stored at 4 ’C overnight in Turkey. Blood Transfus. 2012;10:191–3.
6. National AIDS Control Organisation. Standards for Blood Banks & Blood Transfusion Services. Ministry of Health and Family Welfare. New Delhi; 2007. 1–101 p.
7. Sheffield WP, Bhakta V, Jenkins C, Devine D V. Conversion to the buffy coat method and quality of frozen plasma derived from whole blood donations in Canada. Transfusion. 2010;50:1043–9.
8. Dogra M, Sidhu M, Vasudev R, Dogra A. Comparative analysis of activity of coagulation Factors V and VIII and level of fibrinogen in fresh frozen plasma and frozen plasma. Asian J Transfus Sci. 2015;9:6–8.
9. Huh YO, Lichtiger B, Giacco GG, Guinee VF, Drewinko B. Effect of donation time on platelet concentrates and fresh-frozen plasma. An in vitro study. Vox Sang. 1989;56:21–4.
10. Naghadeh HT, Roudkenar MH. A study of the quantity of some stable and labile coagulation factors in fresh-frozen plasma produced from whole blood stored for 24 hours in Iran. Blood Transfus. 2009;7:39–42.
11. Mahida VI, Bhatti A, Gupte SC. Iron status of regular voluntary blood donors. Asian J Transfus Sci. 2008 Jan;2:9–12.
12. Runkel S, Haubelt H, Hitzler W, Hellstern P. The quality of plasma collected by automated apheresis and of recovered plasma from leukodepleted whole blood Stefan. Transfusion. 2005;45:427–32.
Published
2020-10-29
Issue
Vol 7 No 10 (2020)
Section
Original Article

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access at http://opcit.eprints.org/oacitation-biblio.html).