A Study of Correlation Between Dengue Serological Markers and Platelet Count in Ajmer Region

  • Parmendra Pachori Department of Pathology SMS Medical College, Jaipur, Rajasthan, India
  • Govind Kumar Sharma CHC Bichoon, Jaipur II, Rajasthan India
  • Saroj Pachori Department of Pathology SMS Medical College, Jaipur, Rajasthan, India
  • Geeta Pachori Department of Pathology J.L.N. Medical College, Ajmer, Rajasthan, India
Keywords: Dengue, Serological markers, Thrombocytopenia, Immunochromatographic test

Abstract

Background: Dengue is an acute viral infection has emerged as a notable public health problem. Rapid immunochromatographic test and IgM/IgG ELISA has been the mainstay of diagnosis. Combination of NS1 antigen detection along with antibody detection increases the diagnostic rates. Thrombocytopenia begins during febrile phase. Therefore, we tried to evaluate the association of platelet counts against immunochromatographic test (NS1 and IgM/IgG) and IgM ELISA in dengue infections. Methodology: The study of variation in different platelet parameters in dengue fever cases was undertaken in our department over a period of 18 months from July 2017 to December 2018. Inclusion criteria: All patients with clinical features and serologically positive dengue infection included.   Exclusion criteria: Patient’s which are serologically negative dengue and if routine laboratory test suggesting a bacterial, parasite or any viral infection other than dengue infection or any other disease.  CBC was done from all clinically suspected dengue cases. Serum samples were tested for NS1, IgM and IgG by immunochromatography-based test and IgM MAC-Capture ELISA. Platelet counts were obtained from all serologically positive cases. Test results of dengue specific parameters were compared against platelet counts. Results: Of 636 samples tested, 396 were positive for one or more dengue parameters. Of the 396, 312 were positive for NS1, 152 were positive for IgM, 41 were positive for IgG and 354 were positive for IgM ELISA. Thrombocytopenia was consistently associated with one of the serological parameters. Conclusion: It can be concluded from our study that Thrombocytopenia was found in all serological positive dengue cases and showed a significant correlation with serological markers.

References

Gubler DJ, Clark GG. Dengue/dengue hemorrhagic fever: the emergence of a global health problem. Emerg Infect Dis. 1995; 1: 55–7.

Shu PY & Huang JH. Current advances in dengue diagnosis. Clin Diagn Lab Immunol. 2004; 11:642–50.

Ho TS, Wang SM, Lin YS, Liu CC. Clinical and laboratory predictive markers for acute dengue infection. J Biomed Sci. 2013; 20:75.

Mourão MP, Lacerda MV, Macedo VO, & Santos JB. Thrombocytopenia in patients with virus infection in the Brazilian Amazon. Platelets. 2007; 18(8):605-12.

Kalayanarooj S, Chansiriwongs V and Nimmannitya S. Dengue patients at the Children’s Hospital, Bangkok: 1995–1999. Review. Dengue Bulletin. 2002;26: 33–43.

Shah GS, Islam S, & Das BK. Clinical and laboratory profile of dengue infection in Children. Kathmandu Univ Med J. 2006; 4(1):40-3.

Llamas RR, Ricardo A. Chaín-Castro , Llanes J M, Zepeda MGR, Alvarez TG, et al. Haemorrhagic manifestations in dengue. Rev Biomed. 2005;16: 87-94.

Itoda I, Masuda G, Suganuma A, Imamura A, Ajisawa A, Yamada K et al. Clinical features of 62 imported cases of dengue fever in Japan. Am J Trop Med Hyg. 2006;75(3):470–4.

Pervin M, Tabassum S, Ali MM, Mamun KZ & Islam MN. Clinical and Laboratory Observations associated with the 2000 Dengue Outbreak in Dhaka, Bangladesh. Dengue Bulletin. 2004;28:96-106.

Banerjee M, Chatterjee T, Choudhary GS, Srinivas V, Kataria VK. Dengue: A Clinicohaematological Profile. Med J Armed Forces India. 2008; 64(4):333-336.

Villar-Centeno LA, Díaz-Quijano FA, Martínez-Vega RA. Biochemical alterations as markers of dengue hemorrhagic fever. Am J Trop Med Hyg. 2008;78(3):370-4.

Lin CF, Lei HY, Liu CC et al. Generation of IgM anti-platelet autoantibody in dengue patients. J Med Virol. 2001. 63(2): 143-9.

Sindhanai V, Sageera B, Rajkumar N, & Suresh C, Evaluation of Correlation between Dengue Serological Markers and Platelet Count. Sch J App Med Sci 2016;4(2D):618-622.

Tathe S, Chincholkar VV, Kulkarni DM, Nilekar SL, Ovhal RS, Halgarkar CS. A study of NS1 antigen and platelet count for early diagnosis of dengue infection. Int J Curr Microbiol App Sci 2013;2(12):40-44.

Kanthikar, S. & Kalshetti, V. Correlation of thrombocytopenia and serological markers in early diagnosis of dengue infection with special reference to NS1 antigen. Indian Journal of Pathology and Oncology. 2016. 3. 437-439.

Mehta KD, Ghedia B, Sheth S, Khandhediya S, Shingala H, Sinha M. Study of Correlation Between Platelet Count and Serological Markers of Dengue Infection with Importance of NS1 Antigen in Western Region of India National Journal of Laboratory Medicine. 2016, Vol 5(2): 55-59.

Jyothi P,& Metri BC Correlation of serological markers and platelet count in the diagnosis of Dengue virus infection. Adv Biomed Res. 2015, 30;4:26.

Gupta N., & Bareja R., Diagnosis utility of various serological markers including NSI antigen for timely detection of dengue virus infection. IP International Journal of Medical Microbiology and Tropical Diseases, January-March, 2019;5(1):37-40.

Ingale SV, Upadhye AJ, Upadhye JJ. Correlation of serological markers and thrombocytopenia in early diagnosis of dengue infection. Int J Res Med Sci. 2018;6(3):812-816.

Kulkarni, RD, Patil SS, Ajantha GS, Upadhya AK, Kalabhavi AS, Shubhada RM et al. Association of platelet count and serological markers of dengue infection importance of NS1 antigen. Indian J Med Microbiol, 2011;29(4):359-62.

Joshi AA, Hematological Parameters in Dengue: The Serological Angle A Study. Int. J. Hematol Res 2018. 4(1): 180-184.

Published
2022-03-05
Section
Original Article