Bacteriological Profiles of Semen Culture in Male Patients Having Primary Infertility, Attending Mombasa Assisted Reproduction Centre

  • Uday Dineshchandra Thanki Mount Kenya University, Thika, Kenya/ Mombasa Assisted Reproduction Centre, Mombasa, Kenya
  • Stanley King'e Waithaka Mount Kenya University, Thika, Kenya
  • Mbaruk Abdalla Suleiman Mount Kenya University, Thika, Kenya
  • Mahesh Narbheram Chudasama Mombasa Assisted Reproduction Centre, Mombasa, Kenya
Keywords: Male Infertility, Semen Culture, Bacteriospermia, Antimicrobial Susceptibility Patterns


Background: Infertility is of global health concern affecting one out of six couples worldwide, and Africa has reported the highest rates. Bacterial semen infections are considered to be one of the significant causes of infertility. Effective antimicrobial therapy is therefore essential in treatment of bacteriospermia. This study aimed to identify the bacterial pathogens present in semen and involved performing the antimicrobial susceptibility testing to improve the clinical management by identifying the antimicrobials that can be the best treatment option.Methods: Ninety semen specimens were collected from male patients having primary infertility who attended the clinic. Semen was cultured on Nutrient agar, Blood agar, MacConkey agar and Chocolate blood agar using standard bacteriological techniques. Bacterial isolates identification was done by studying the colony characteristics, performing gram staining reactions and biochemical testing. Modified Kirby-Bauer method was used in performing the antimicrobial susceptibility testing.Result: 51 (56.67%) patients presented with bacteriospermia. Staphylococcus aureus (29.41%) was the most common bacteria isolated, followed by Escherichia coli (23.53%), Coagulase negative staphylococcus species (17.65%), Klebsiella pneumoniae (11.76%), Proteus. mirabilis (7.84%), Pseudomonas. aeruginosa (5.88%) and Neisseria gonorrhoeae (3.92%). Most of the bacteria isolated were highly sensitive to ciprofloxacin, cefotaxime and ofloxacin and moderately sensitive to amikacin, gentamycin and cefuroxime.Conclusion: Bacteriospermia prevalence rate was 56.67% and was commonly observed in the age group of 30 - 39 years. Staphylococcus aureus was found to be the predominant causative agent of semen infection. The most effective antimicrobials were ciprofloxacin, cefotaxime and ofloxacin and the antimicrobial with least activity was co-trimoxazole.


Inhorn, M. C., & Patrizio, P. (2015). Infertility around the globe: new thinking on gender, reproductive technologies and global movements in the 21st century. Human reproduction update, 21(4), 411–426.

Cariati, F., D'Argenio, V., & Tomaiuolo, R. (2019). The evolving role of genetic tests in reproductive medicine. Journal of translational medicine, 17(1), 267.

Ekhaise, F. O., & Richard, F. R. (2011). Common bacterial isolates associated with semen of men attending the fertility clinic of the university of Benin teaching hospital (U.B.T.H), Benin City, Nigeria. African Journal of Microbiology Research, 5, 3805–3809

Sengupta, P., Borges, E., Jr, Dutta, S., & Krajewska-Kulak, E. (2018). Decline in sperm count in European men during the past 50 years. Human & experimental toxicology, 37(3), 247–255.

Menkveld, R., Wong, W. Y., Lombard, C. J., Wetzels, A. M., Thomas, C. M., Merkus, H. M., & Steegers-Theunissen, R. P. (2001). Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in-vivo thresholds. Human reproduction (Oxford, England), 16(6), 1165–1171.

Altmäe, S., Franasiak, J. M., & Mändar, R. (2019). The seminal microbiome in health and disease. Nature reviews. Urology, 16(12), 703–721.

Tomaiuolo, R., Veneruso, I., Cariati, F., & D'Argenio, V. (2020). Microbiota and Human Reproduction: The Case of Male Infertility. High-throughput, 9(2), 10.

Lundy, S. D., Vij, S. C., Rezk, A. H., Cohen, J. A., Bajic, P., & Ramasamy, R. (2020). The microbiome of the infertile male. Current opinion in urology, 30(3), 355–362.

Dinah Moraa, Dr. Scholastica Mathenge, Arodi Washington, Torome Tom, Oliver Mbuthia, Martin Kinyua (2019). Antibiotic Susceptibility Pattern among Male Patients with Urinary Tract Infection in Special Treatment Centre, Nairobi County, Kenya. Int. J. Adv. Multidiscip. Res. 6(2): 36-41.

Vos, P., Garrity, G., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.-H. & Whitman, W.B. (2009). Bergey’s Manual of Systematic Bacteriology, 2nd ed., vol. 3, Springer-Verlag, New York, NY

Clinical and Laboratory Standards Institute (CLSI) (2018) Performance Standards for Antimicrobial Susceptibility Testing. 28th Edition. CLSI supplement M100

Granato PA, Morton V, Morello JA. (2019) Laboratory Manual and Workbook in Microbiology applications to patient care 12th Edition, McGraw-Hill Education pages 82-84.

Fraczek, M., & Kurpisz, M. (2015). Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen. Folia histochemica et cytobiologica, 53(3), 201–217.

Nwofor C. N, Ogbulie J. N, Obiajuru I. O. C and Elezua N (2016). Antibiotic susceptibility pattern of bacterial isolated from semen of men with fertility problem in Imo State, Nigeria. African Journal of Medicine and Medical Sciences. 4. 020 - 023.

Nasrallah, Y., Anani, M., Omar, H., Hashem, A. (2018). Microbiological profiles of semen culture in male infertility. Human Andrology, 8(2), 34-42.

Al-Jebouri, M. and Mdish, S. (2019) Tracing of Antibiotic-Resistant Bacteria Isolated from Semen of Iraqi Males with Primary Infertility. Open Journal of Urology, 9, 19-29.

Zeyad, A., Hamad, M. F., & Hammadeh, M. E. (2018). The effects of bacterial infection on human sperm nuclear protamine P1/P2 ratio and DNA integrity. Andrologia, 50(2)

Vilvanathan, S., Kandasamy, B., Jayachandran, A. L., Sathiyanarayanan, S., Tanjore Singaravelu, V., Krishnamurthy, V., & Elangovan, V. (2016). Bacteriospermia and Its Impact on Basic Semen Parameters among Infertile Men. Interdisciplinary perspectives on infectious diseases, 2016, 2614692.

Isaiah, I. N., Nche, B. T., Nwagu, I. G., & Nnanna, I. I. (2011). Current studies on bacterospermia the leading cause of male infertility: a protégé and potential threat towards mans extinction. North American journal of medical sciences, 3(12), 562–564.

Sanocka-Maciejewska, D., Ciupińska, M., & Kurpisz, M. (2005). Bacterial infection and semen quality. Journal of reproductive immunology, 67(1-2), 51–56.

Nasiru A S, Farouk S N, Sani U D, Muhammad A. (2018) Antibiotic Sensitivity Pattern of Bacteria Isolated from Semen of Male Patients with Infertility Attending Murtala Muhammad Specialist Hospital Kano, Nigeria. Advancements in Bioequivalence & bioiavailability. 1(4). ABB.000519.2018

Gupta, K., Hooton, T. M., & Stamm, W. E. (2001). Increasing antimicrobial resistance and the management of uncomplicated community-acquired urinary tract infections. Annals of internal medicine, 135(1), 41–50.

Jensen, S. O., & Lyon, B. R. (2009). Genetics of antimicrobial resistance in Staphylococcus aureus. Future microbiology, 4(5), 565–582.

Miró, E., Navarro, F., Mirelis, B., Sabaté, M., Rivera, A., Coll, P., & Prats, G. (2002). Prevalence of clinical isolates of Escherichia coli producing inhibitor-resistant beta-lactamases at a University Hospital in Barcelona, Spain, over a 3-year period. Antimicrobial agents and chemotherapy, 46(12), 3991–3994.

Matzov, D., Bashan, A., & Yonath, A. (2017). A Bright Future for Antibiotics? Annual review of biochemistry, 86, 567–583.

Khan S, Singh P, Siddiqui Z, Ansari M. Pregnancy-associated asymptomatic bacteriuria and drug resistance. Journal of Taibah University Medical Sciences. 2015 Sep 1;10(3):340-5.

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