RBC Histogram as supplementary diagnostic tool with peripheral smear examination in evaluating anemias
Keywords:
RBC Histogram, Anaemia, Blood indices, Red cell distribution width.
Abstract
Background: Red blood cell(RBC) histogram provide an idea about morphological changes of red blood cells in hematological disorders. Peripheral smear examination findings are usually correlated with complete blood cell counts by automated analyzer. To known the utility and advantage of red cell histogram and correlation of microscopic examination of peripheral smear with automated histogram pattern. Methods: Blood sample was collected from 220 anemia patients in ethylene diamine tetra acetic acid (EDTA) tubes for peripheral smear examination and ran in Beckman coulter LH 780 automated hematology analyzer for obtaining histogram, complete blood count includes hemoglobin, total leucocyte count, Platelet count, red blood cell indices and red cell distribution width. This study was undertaken for a period of one month of November 2016 in department of pathology, central laboratory, narayana medical college & hospital, Nellore. Results: This study of histograms of 220 different types of anemia consisted predominantly females 154 (70%) , and males 64(30%) . Maximum cases of anemia were noted in 30-40 years of age range. Microcytic hypo chromic anemia was the most common (63.63%) followed by normocytic normochromic anemia (19.4%), macrocytic hypochromic anemia (2.2%), dimorphic anemia (12.72%) and pancytopenia (1.8%).Left shifted curve and broad base mostly seen in microcytic anemia, right shift curve seen in macrocytic anemia and bimodal peak mostly seen in dimorphic anemia.Conclusion: Histogram can be used as an important screening test for hematology and can become a new parameter in the diagnosis of anemia though peripheral examination remains the definitive diagnostic test for evaluation.DOI: 10.21276/APALM.1468References
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4. Bessman JD. Red bllod cell fragmentation: Improved detection and identification of causes.Am j clin pathol.1988:90(3):268-73
5. Sandhya I, Muhasin T.P. Study of RBC Histogram in various anemias. Journal of Evolution of Medical and Dental sciences 2014; 3( 74),15521-34
6. Chavda J, Goswami P, Goswami A. RBC histogram as diagnostic tool in anemias. IOSR Journal of Dental and Medical Sciences .2015;14(10), 19-22
7. Benie T. Constantino SH. The Red Cell Histogram and The Dimorphic Red Cell Population .LAB MEDICINE.2011;42(5):300-8
8. Brigden ML. A Systemic approach to macrocytosis: sorting out the causes. Postgrad Med. 1995;97(5):171-84.
9. Kaferle J, Strzoda CE. Evaluation of macrocytosis. Am Fam Physician. 2009; 79(3): 203-8.
10. Argento V, Roylance J, Skudlarska B, et al. Anemia prevalence in a home visit geriatric populations. J Am Med Dir Assoc, 2008;9(6):422-6
11. Younis M, Daugher GA, Dulanto JV, Njeim M, Kuriakose P. Unexplained macrocytosis. South Med J.2013;106(2):121-5
12. McNamee T, Hyland T, Harrington J, Cadogan S, Honari B, Perera K, et al. Haematinic deficiency and macrocytosis in middle-aged and older adults. PLoS One. 2013; 8 (11): e77743
2. Fossat C, David M, Harle JR, Sainty D, Horschowski N, Verdot JJ, Mongin M et al. New parameters in erythrocyte counting value of histograms. Archpathol Lab Med. 1987;111(12):1150-54
3. Gulati GL, Hyun BH; The Automated CBC. A current perspective. HematoloncolClin North Am.1994;8(4):593-603
4. Bessman JD. Red bllod cell fragmentation: Improved detection and identification of causes.Am j clin pathol.1988:90(3):268-73
5. Sandhya I, Muhasin T.P. Study of RBC Histogram in various anemias. Journal of Evolution of Medical and Dental sciences 2014; 3( 74),15521-34
6. Chavda J, Goswami P, Goswami A. RBC histogram as diagnostic tool in anemias. IOSR Journal of Dental and Medical Sciences .2015;14(10), 19-22
7. Benie T. Constantino SH. The Red Cell Histogram and The Dimorphic Red Cell Population .LAB MEDICINE.2011;42(5):300-8
8. Brigden ML. A Systemic approach to macrocytosis: sorting out the causes. Postgrad Med. 1995;97(5):171-84.
9. Kaferle J, Strzoda CE. Evaluation of macrocytosis. Am Fam Physician. 2009; 79(3): 203-8.
10. Argento V, Roylance J, Skudlarska B, et al. Anemia prevalence in a home visit geriatric populations. J Am Med Dir Assoc, 2008;9(6):422-6
11. Younis M, Daugher GA, Dulanto JV, Njeim M, Kuriakose P. Unexplained macrocytosis. South Med J.2013;106(2):121-5
12. McNamee T, Hyland T, Harrington J, Cadogan S, Honari B, Perera K, et al. Haematinic deficiency and macrocytosis in middle-aged and older adults. PLoS One. 2013; 8 (11): e77743
Published
2017-12-19
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