Role of Immunohistochemistry in trephine biopsies of Bone Marrow: A 5-year Retrospective study from a tertiary care hospital

  • Ravi Teja J Sri Ramachandra Medical College & Research Institute.
  • Febe Renjitha Suman Sri Ramachandra Medical College & Research Institute.
  • Lenna Dennis Joseph Sri Ramachandra Medical College & Research Institute.
  • Jerusha Samuela Jacob Sri Ramachandra Medical College & Research Institute
  • Rithika Rajendran Sri Ramachandra Medical College & Research Institute.
  • Jesu Magdalene S Sri Ramachandra Medical College & Research Institute
  • Sai Shalini CN Sri Ramachandra Medical College & Research Institute
Keywords: Bone marrow, immunohistochemistry, leukemia, metastasis, trephine biopsy

Abstract

Background: Bone Marrow Biospy (BMB) often needs ancillary tests like Immunohistochemistry (IHC) to confirm the morphological diagnosis, to categorize malignant conditions.Methods: A retrospective study was done on BMB with IHC done on the sections are reviewed and analysed correlating the clinical, Bone marrow aspirate(BMA) and BMB interpretations.Result: A 5 year study on 934 BMB required IHC as an adjunct on 16.2% of the biopsies. It was done on 10.6% of cases. The distribution of cases was 43% of Acute leukemia(AL), 19% multiple myeloma, 8& lymphomas, 9% metastasis. 20 % of other cases which included chronic lymphocytic leukemia(CLL), myelodysplastic syndrome (MDS), megaloblastic anemias, infective conditions and reactive marrows.Conclusion: IHC is a reliable method to confirm and categorize AL, to differentiate reactive and neoplastic plasmacytosis, to confirm, categorize and stage lymphomas, to detect metastasis. DOI: 10.21276/APALM.1506

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Published
2017-12-21
Issue
Vol 4 No 6 (2017)
Section
Original Article

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