Study of Acid Phosphatase and Heamoglobin Level in Malarial and Non Malarial Fever Patients
Keywords:
Acid phosphatase, Malaria, Hemoglobin
Abstract
Background: Malaria is a mosquito borne disease. It is a major health hazard in India and other tropical countries. An estimated 198 million cases of malaria and 584 000 malaria deaths occurred in 2013. Acid phosphatase (ec 3.1.3.2, ACP) includes all the phosphatases with optimal activity below the pH of 7.0. ACP is present in lysosomes in all cells and exist extra-lysosomally in erythrocytes. Little is known about the levels of ACP in infectious diseases like malaria. So we undertook this study to know the Hb and ACP level in malarial patients.Methods: 60 subjects in the age group of 20 to 50 years were included in this study. These subjects were divided into 2 groups. Group 1 included 30 confirmed malaria fever patients. Group 2 included 30 non-malarial fever patients of same age & sex group. These selected subjects had no prostate problems, previous anaemia, or any other kind of bone disorder. Serum ACP was estimated using kit methodResult: The serum ACP levels were significantly increased in malarial fever patients when compared with the non malaria fever patients (P=0.032). There is also statistical significant difference in the Hb levels between malarial and non-malarial fever patientsConclusion: Our results of increased levels of serum ACP and decreased Hb in malarial fever patients could be used as a marker for haemolysis & anemia. ACP may be used as an additional investigation in the diagnosis of malaria.DOI:10.21276/APALM.1515References
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15. Pratinidhi SA, Agarwal SV, Sagare AA, Gawade SH, Haribhakta SV, Hegde MV, et al. Study of serum acid phosphatase levels in children with malaria. IJHSR. 2014;4(2):73-7.
16. Prassannachandra, D’Souza V, D’Souza B. Comparative study on lipid peroxidation and antioxidants vitamins E & C in falciparum and vivax malaria. Ind J Clin Biochem. 2006;21(2):103–106.
17. Goldberg DE, Slater AF, Cerani A, Henderson GB. Hemoglobin degradation in malaria parasite Plasmodium falciparum: anordered process in a unique organelle. Proc Natl Acad Sci. 1990;87(8):2931–2935.
18. D’Souza B, d’souzav, Swagata H,Vijayalaxmi K, Namratha A.S. Erythrocyte antioxidant enzymes and their correlation with malondialdehyde in malaria. Biochemical research 2009; 20(1): 25-27.
19. Gupta CM. Red cell membrane alterations in malaria. Ind J Biochem Biophys. 1988;25:20–4.
20. Bhabhani SD, Nilini KN. Evidence for erythrocyte lipid peroxidation in acute falciparum malaria. Trans R Soc Trop Med Hyg.1999;93:58–62
2. Rathod Sangita. Study of sodium and potassium ion disturbances in malaria at Ahmedabad, Gujarat, India. International Archives of Integrated Medicine, 2014;1(1):7-11.
3. Rajkumar A., Rao S., Sundaram S. Clinical outcome in Malaria-Reiterating the role of parasitic index. Indian Journal of Clinical Practice 2012; 22(9):450-453.
4. World malaria report 2014 World Health Organization.
5. Burtis, Ashwood, Bruns. Tietz Fundamentals of Clinical Chemistry. 6th ed. In:Enzymes. Pennsylvania:Saunders An imprint of Elsevier; 2012; p. 334-335.
6. D’Souza B, • Parthasarathy R, D’Souza V. Acid Phosphatase as a Marker in Malaria. Ind J Clin Biochem 2011; 26(4):396–399
7. Kanwar G., Yadav M., et al. Elevated serum acid phosphatase: prospective malarial marker. International Journal of Research in Applied,Natural and Social Sciences 2014; 2(9):11-14.
8. Vasudevan DM, Sreekumari S. Text book of biochemistry. 3rd ed. New Delhi: Jaypee Brothers Medical Publishers (P) Limited; 2001. p. 50–2.
9. El-Ishaq A, Omoluabi T. O. Plasma activity of tartrate resistant acid phosphatase in acute malaria infection International Journal of Life Science and Engineering. 2015; 1(4): 183-188.
10. Calibreath, D. F. (1992). Chemical Chemistry. W.B Sanders. Philadelphia U.S.A. P. 162
11. Zilva, J.F. Pannal, P.R. and Mayane, P.D.(1988).Clinical chemistry in diagnosis and treatment.5th edition. Loyd-Luke.London.pp.454.
12. Bottini E, Gloria-Bottinin F, Borgiani P, et al. ACP1 and human adaptability. Association with common diseases: a case-control study. Hum Genet 1995;96:629–37.
13. Bottini E, Lucarelli P, Agostino R, et al. Favism: association with erythrocyte acid phosphatase phenotype. Science 1971;171:409–11.
14. Garba, Gatsing D, Uborn G; Elevated total andisoenzyme forms of acid phosphatase in falciparum malaria. Comput Rendus Biol.2006;329(2):75-8.
15. Pratinidhi SA, Agarwal SV, Sagare AA, Gawade SH, Haribhakta SV, Hegde MV, et al. Study of serum acid phosphatase levels in children with malaria. IJHSR. 2014;4(2):73-7.
16. Prassannachandra, D’Souza V, D’Souza B. Comparative study on lipid peroxidation and antioxidants vitamins E & C in falciparum and vivax malaria. Ind J Clin Biochem. 2006;21(2):103–106.
17. Goldberg DE, Slater AF, Cerani A, Henderson GB. Hemoglobin degradation in malaria parasite Plasmodium falciparum: anordered process in a unique organelle. Proc Natl Acad Sci. 1990;87(8):2931–2935.
18. D’Souza B, d’souzav, Swagata H,Vijayalaxmi K, Namratha A.S. Erythrocyte antioxidant enzymes and their correlation with malondialdehyde in malaria. Biochemical research 2009; 20(1): 25-27.
19. Gupta CM. Red cell membrane alterations in malaria. Ind J Biochem Biophys. 1988;25:20–4.
20. Bhabhani SD, Nilini KN. Evidence for erythrocyte lipid peroxidation in acute falciparum malaria. Trans R Soc Trop Med Hyg.1999;93:58–62
Published
2018-02-27
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