Significance of Ki-67 in prognostication of soft tissue tumors
Keywords:
FNCLCC grading, immunohistochemical staining, Ki-67 Index, soft tissue tumors
Abstract
Background: Histological grading is the most important prognostic factors and the best indicator of metastatic risk in adult soft tissue sarcomas. It has been shown that Ki67 immunohistochemical (IHC) staining is an effective method of assessing the prognosis in a number of tumor types. Ki67 is a cell cycle antigen which is elevated in proliferation states. High level of Ki67 expression is an independent prognostic indicator that correlates with poor outcome in patients with sarcomas.Methods: In this study 53 cases of soft tissue tumor were selected and were classified according to FNCLCC system. IHC staining of Ki-67 was done in all the 53 samples. Diagnosis was made mainly based on histopathological pattern analysis and with the use of IHC.Results: By using FNCLCC grading soft tissue tumor cases were graded as grade I, II and III. The correlation between the FNCLCC grading and Ki67 was done and it was observed that there was statistically significant positive correlation between Ki67 and the grade of the tumor, as evidenced by P- values of 0.002, 0.005 and 0.004. The correlation between the 5 FNCLCC grading and Ki67 index was also assessed. Statistically significant positive correlation between Ki67 index and the grade of the tumor was observed.Conclusion: There was significant correlation noted between FNCLCC grading and Ki-67 index. Thus it can be recommended that Ki-67 IHC stain should be done on routine basis to accurately grade the sarcomas so that it will be beneficia l for the management of the patient.DOI:10.21276/APALM.1550References
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2.Jean-Michel Coindre et al. Grading of Soft Tissue Sarcomas. Review and Update. Arch Pathol Lab Med. October 2006 ;130: 1448-1453.
3.Coindre JM, Terrier P, Bui NB, Bonichon F, Collin F, Le D, V, Mandard AM, et al. Prognostic factors in adult patients with locally controlled soft tissue sarcoma. A study of 546 patients from the French Federation of Cancer Centers Sarcoma Group. J Clin Oncol. 1996; 14: 869-877.
4.Coindre JM, Terrier P, Guillou L, Le D, V, Collin F, Ranchere D, et al. Predictive value of grade for metastasis development in the main histologic types of adult soft tissue sarcomas: a study of 1240 patients from the French Federation of Cancer Centers Sarcoma Group. Cancer. 2001; 91: 1914-1926
5.Neuville A et al.Grading of soft tissue sarcomas: from histological to molecular assessment. The journal of royal college of pathologist of Australia. 2014; 46( 2): 113–120.
6.Oliveira AM, Nascimento AG.Grading in soft tissue tumors: principles and problems. Springer-Verlag. October 2001; 30(10): 543–559.
7.Guillou L, Coindre JM, Bonichon F, Nguyen BB, Terrier P, Collin F, et al. Comparative study of the National Cancer Institute and French Federation of Cancer Centers Sarcoma Group grading systems in a population of 410 adult patients with soft tissue sarcoma. J Clin Oncol. 1997; 15: 350-362.
8.Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol2000; 182:311–22.
9.Jacquemier JD, Penault Llorca FM, Bertucci F, et al: Angiogenesis as a prognostic marker in breast carcinoma with conventional adjuvant chemotherapy: a multiparametric and immunohistochemical analysis. J Pathol. 1998; 184: 130-135.
10.Kausch I, Lingnau A, Endl E, et al: Antisense treatment against Ki-67 mRNA inhibits proliferation and tumor growth in vitro and in vivo. Int J Cancer. 2003; 105: 710-716.
11.Liu J, Fang L, Cheng Q, et al: Effects of G250 promoter controlled conditionally replicative adenovirus expressing Ki67-siRNA on renal cancer cell. Cancer Sci. 2012; 103: 1880-1888.
12.Karamitopoulou E, Perentes E, Tolnay M and Probst A: Prognostic significance of MIB1, p53, and bcl2 immunoreactivity in meningiomas. Hum Pathol.1998; 29: 140-145.
13.Geyer FC, Rodrigues DN, Weigelt B and Reis Filho JS: Molecular classification of estrogen receptor positive/luminal breast cancers. Adv Anat Pathol. 2012; 19: 39-53.
14.Claudio PP, Zamparelli A, Garcia FU, et al: Expression of Cell Cycle regulated Proteins pRb2/p130, p107, p27kip1, p53, mdm2, and Ki67 (MIB1) in Prostatic Gland Adenocarcinoma1.Clin Cancer. 2002; 8:1808-15.
15.HY Hu, Liu H, JW Zhang, et al: Clinical significance of Smac and Ki-67 expression in pancreatic cancer. Hepato-gastroenterology.2012; 59: 2640-2643.
16.Golouh R, Bracko M, Novak J. Predictive value of proliferation related markers p53 and DNA ploidy for survival in patients with soft tissue spindle cell sarcomas. Mod. Pathol. 1996; 9: 919-924.
17.Hoos A, Stojadinovic A, Mastorides S et al. High Ki-67 Proliferative Index Predicts Disease Specific Survival in Patients with High- Risk Soft Tissue Sarcomas. Cancer 2001; 92: 869-74.
18.Lian Tao Li, Guan Jiang, Qian Chen, Jun Nian Zheng. Ki67 is a promising molecular target in the diagnosis of cancer (Review). Molecular Medicine Reports. 2011; 11: 1566-1572.
2.Jean-Michel Coindre et al. Grading of Soft Tissue Sarcomas. Review and Update. Arch Pathol Lab Med. October 2006 ;130: 1448-1453.
3.Coindre JM, Terrier P, Bui NB, Bonichon F, Collin F, Le D, V, Mandard AM, et al. Prognostic factors in adult patients with locally controlled soft tissue sarcoma. A study of 546 patients from the French Federation of Cancer Centers Sarcoma Group. J Clin Oncol. 1996; 14: 869-877.
4.Coindre JM, Terrier P, Guillou L, Le D, V, Collin F, Ranchere D, et al. Predictive value of grade for metastasis development in the main histologic types of adult soft tissue sarcomas: a study of 1240 patients from the French Federation of Cancer Centers Sarcoma Group. Cancer. 2001; 91: 1914-1926
5.Neuville A et al.Grading of soft tissue sarcomas: from histological to molecular assessment. The journal of royal college of pathologist of Australia. 2014; 46( 2): 113–120.
6.Oliveira AM, Nascimento AG.Grading in soft tissue tumors: principles and problems. Springer-Verlag. October 2001; 30(10): 543–559.
7.Guillou L, Coindre JM, Bonichon F, Nguyen BB, Terrier P, Collin F, et al. Comparative study of the National Cancer Institute and French Federation of Cancer Centers Sarcoma Group grading systems in a population of 410 adult patients with soft tissue sarcoma. J Clin Oncol. 1997; 15: 350-362.
8.Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol2000; 182:311–22.
9.Jacquemier JD, Penault Llorca FM, Bertucci F, et al: Angiogenesis as a prognostic marker in breast carcinoma with conventional adjuvant chemotherapy: a multiparametric and immunohistochemical analysis. J Pathol. 1998; 184: 130-135.
10.Kausch I, Lingnau A, Endl E, et al: Antisense treatment against Ki-67 mRNA inhibits proliferation and tumor growth in vitro and in vivo. Int J Cancer. 2003; 105: 710-716.
11.Liu J, Fang L, Cheng Q, et al: Effects of G250 promoter controlled conditionally replicative adenovirus expressing Ki67-siRNA on renal cancer cell. Cancer Sci. 2012; 103: 1880-1888.
12.Karamitopoulou E, Perentes E, Tolnay M and Probst A: Prognostic significance of MIB1, p53, and bcl2 immunoreactivity in meningiomas. Hum Pathol.1998; 29: 140-145.
13.Geyer FC, Rodrigues DN, Weigelt B and Reis Filho JS: Molecular classification of estrogen receptor positive/luminal breast cancers. Adv Anat Pathol. 2012; 19: 39-53.
14.Claudio PP, Zamparelli A, Garcia FU, et al: Expression of Cell Cycle regulated Proteins pRb2/p130, p107, p27kip1, p53, mdm2, and Ki67 (MIB1) in Prostatic Gland Adenocarcinoma1.Clin Cancer. 2002; 8:1808-15.
15.HY Hu, Liu H, JW Zhang, et al: Clinical significance of Smac and Ki-67 expression in pancreatic cancer. Hepato-gastroenterology.2012; 59: 2640-2643.
16.Golouh R, Bracko M, Novak J. Predictive value of proliferation related markers p53 and DNA ploidy for survival in patients with soft tissue spindle cell sarcomas. Mod. Pathol. 1996; 9: 919-924.
17.Hoos A, Stojadinovic A, Mastorides S et al. High Ki-67 Proliferative Index Predicts Disease Specific Survival in Patients with High- Risk Soft Tissue Sarcomas. Cancer 2001; 92: 869-74.
18.Lian Tao Li, Guan Jiang, Qian Chen, Jun Nian Zheng. Ki67 is a promising molecular target in the diagnosis of cancer (Review). Molecular Medicine Reports. 2011; 11: 1566-1572.
Published
2017-10-30
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