Seropositivity of Hepatitis B Surface Antigen (HBsAg) among blood donors at the blood bank of a tertiary care hospital

  • Asha Purohit Dept. of Pathology,GCS Medical College, Hospital and Research centre, Ahmedabad, Gujarat, India
  • Puja Jarwani Dept. of Pathology,GCS Medical College, Hospital and Research centre, Ahmedabad, Gujarat, India
  • S. M. Patel Dept. of Pathology,GCS Medical College, Hospital and Research centre, Ahmedabad, Gujarat, India
Keywords: Hepatitis B Surface Antigen, Seroprevalence, Blood donors.


Background: Blood is a scarce, but life saving resource. Hepatitis B virus (HBV) is transmitted by blood and blood products. Hence screening of hepatitis B surface antigen (HBsAg) among blood donors is important for safe blood transmission. The reports about the prevalence of HBV in the blood donors of Gujarat are limited and this study was conducted with an aim to find the seropositivity of HBV in the blood donors in a tertiary care hospital at Ahmedabad. Methods: This is a retrospective analytical study conducted over a period of five years from 1st January 2012 to 31st December 2016. A total of 9353 donor blood samples were screened for HBsAg status using enzyme linked immunosorbent assay (ELISA). Reactive samples were retested in duplicate. The samples reactive in all three tests were considered positive. The samples which were reactive only in first test and non reactive on repeat testing were labeled as false positive. The samples reactive in any one of repeat testing were considered as positive. Results: Out of 9353 blood donors screened 137(1.46 %) donors were initially reactive and 70(0.75 %) donors were reactive after triple testing. The observed seroprevalence of HBsAg was higher in replacement donors than in voluntary donors (0.60% vs. 0.15%, respectively). 100%seroreactivity was in male donors and no seroreactivity was observed in female donorsConclusions: Our study showed similar HBsAg seroprevalence as reported by World Health Organization (WHO) statistics in low prevalence zone (less than 2%).DOI:10.21276/APALM.1653

Author Biography

Asha Purohit, Dept. of Pathology,GCS Medical College, Hospital and Research centre, Ahmedabad, Gujarat, India
tutor in pathology


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