MicroRNAs versus traditional biomarkers in predicting reperfusion injury in Hepatitis C Patients after major surgery

  • Hala Mourad Demerdash Pharos University in Alexandria Alexandria University Hospitals,Egypt
  • Emad Arida Alexandria Faculty of Medicine
  • Ola Zanaty Alexandria Faculty of Medicine
  • Alaa Dawood Faculty of Medicine, Menoufiya University
Keywords: miRNA, chronic hepatitis C, Ischemia, reperfusion, propofol, sevoflurane.


Background:  Exposure to anesthesia in patients with chronic hepatitis C (CHC) may induce hepatic ischemia / reperfusion (I/R)  with subsequent biochemical changes, provoked by alteration in molecular processes. This study explored miR-122 and miR-223 expression changes induced by anesthesia in CHC patients and their role in predicting  hepatic I/R injury. Methods: This study included two hundered ASA II - III adult patients, scheduled for major surgery under general anesthesia.  Patients were divided into 2 main groups; CHC patients group and non-hepatitis patients group. Each group was further divided into 2 equal  subgroups  (50 patients each) according to type of anesthesia received either Sevoflurane SC /S or Propofol PC/P ). Blood samples were analyzed for liver enzymes, prothrombin time, and serum lactate at several intervals; preoperative, 12 and 48 hours postoperative. Also, plasma miR-122 and miR-223 were estimated by Reverse Transcription PCR preoperative and 12 hours postoperative.Results: CHC groups showed significantly increased preoperative expression of both miR-122 and miR-223 compared to non-hepatitis groups (P<0.05). Moreover, postoperative samples revealed more exaggerated expression miR-122 and miR-223. Receiver operating characteristic curve (ROC) results revealed;values for area under the ROC for miR-122, miR-223, ALT and serum Lactate were 0.991, 0.965, 0.746 and 0.732 respectively. Based on the ROC finding, for prediction IR in CHC patients receiving anesthesia miR-122 had best Accuracy, sensitivity, and specificity.Conclusion: Our results  suggested that both miR-122 and miR-223 could be regarded as more sensitive and specific biomarkers for predicting hepatic I/R injury than traditional biomarkers particularly in CHC patients undergoing major surgery. DOI: 10.21276/APALM.1718

Author Biography

Ola Zanaty, Alexandria Faculty of Medicine


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