Correlation of bone marrow aspiration, touch imprint findings and bone marrow biopsy findings in pancytopenia.

  • R Sindhu Berhampur university, Odisha
  • Pramita Sahu Berhampur University
  • Debi Prasad Mishra Berhampur University
  • Samir Kumar Behera Berhampur university
Keywords: Pancytopenia, Bone Marrow Aspiration, Bone Marrow Biopsy, Bone Marrow Imprint


Background: Pancytopenia is an important hematological entity encountered in our day to day clinical practice.Bone marrow examination is a useful and cost effective diagnostic approach in evaluating these disorders. Although bone marrow aspiration alone is sufficient in diagnosing most of these disorders, the comparative evaluation of bone marrow aspiration (BMA), imprint (BMI) and biopsy (BMB) is needed for more rapid and efficient diagnosis. Many studies have been done in evaluating the importance of each of these procedures in various hematological disorders, with very few studies evaluating the importance of all three procedures in pancytopenia specifically.So, the objective of our study was to compare and correlate all three procedures in cases of pancytopenia.Methods: All patients who were found to be pancytopenic were evaluated and bone marrow aspiration, imprint and biopsy were done in all the cases simultaneously at the same site using two needle technique.Result: A total of 148 cases of pancytopenia were studied and most common cause of pancytopenia was hypoplastic/aplastic anemia (33.8%), followed by nutritional anemia (30.4%). We found a positive correlation of 72.04% in BMA with BMB and 91.20% in BMI with BMB.Conclusion: Trephine biopsy has greater value in providing information about the architecture, pattern of cellularity, and presence of infiltrates and granulomas. At the same time morphological features of individual cells may be identified by aspiration and by imprint in cases of dry tap. Hence, both BMA and BMB along with imprint cytology are complementary to each other.

Author Biography

R Sindhu, Berhampur university, Odisha
Department of pathology, Postgraduate


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