Annals of Pathology and Laboratory Medicine

Bacteriological Study of Wound Infections and Their Antibiogram Pattern at A Tertiary Care Hospital in Suburban Area of Hyderabad

Upasana Bhumbla — 2018-09-17

Background: Wound infections can be caused through two major sources: exogenous and endogenous bacteria. The probability of wound infection largely depends on the patient’s systemic host defenses, local wound conditions and microbial burden. Despite modern surgical techniques and the use of antibiotic prophylaxis, Surgical Site Infection (SSI) is one of the most common complications encountered in surgery. SSI places a significant burden on both the patient and health system. SSI is thus a major cause of morbidity, prolonged hospital stays and increased health costs.

Methods: Two wound swabs were collected from the wound and from a drop of aspirate, smear was made on clean glass slide and Gram staining was done for direct microscopic examination under oil immersion 100X objective to know various morphological types of bacteria and presence or absence of inflammatory cells. Second swab/drop of aspirate was used for culture by inoculating it on routine media like Blood Agar, Nutrient Agar and MacConkey’s agar, incubated at 37° C for 24 hrs. aerobically.

Result: Out of 108 pus samples, 101(88.5%) were culture positive for bacterial growth and no growth was observed in 7(11.4%) cases. Out of 101 bacterial culture positive cases, 96 were nonbacterial and 5 were poly bacterial. Out of 96 bacterial isolates; S. aureus (38/36.6%) was the commonest followed by P. aeruginosa (26/24.7%)

Conclusion: The study concludes that variety of aerobic bacteria is responsible for wound infection with predominance of Staphylococcus aureus followed by Pseudomonas aeruginosa.

A Case of Clear Cell Sarcoma of Kidney in An Adult: An Extremely Rare Presentation

Pragya Nirav Vakani — 2018-09-17

Clear cell sarcoma of the kidney (CCSK) is a malignant renal tumor of childhood with a propensity to metastasize to bone and other organs. It is extremely rare in adults, must be differentiated from clear cell carcinoma of kidney which is more common in adults. We report a case of CCSK in a 45 year old female, confirmed by Immunohistochemistry (IHC) & managed with radical nephrectomy. The aim of this case report is to increase the awareness about this entity in adults with brief review of relevant literature.

Infarcted Fibroadenoma: An unusual case with its Cyto-histopathological Correlation

Nehal Ahmad — 2018-09-17

Fibroadenoma is a benign breast lesion of adolescents and young women. It constitutes 20 % of all benign breast tumours. Fibroadenoma is associated with many secondary changes like calcification, ossification, hyalinization, myxoid and apocrine squamous metaplasia. Spontaneous infarction in fibroadenoma is very rarely seen. We report one such case of spontaneous infarction of fibroadenoma in a 16 year old unmarried female with its cyto-histopathological correlation, that posed us with diagnostic dilemma in early stage. The possibility of this entity should be considered while diagnosing the breast lumps on cytology. The esion was further confirmed on histology.

Cytological Diagnosis of an Uncommon Cause of Post-Menopausal Vaginitis

Manjari Kishore — 2018-09-17