Comparison of ELISA & rapid screening tests for the diagnosis of HIV, HBV & HCV among blood donors in blood bank of C.C.M. Medical College Durg Chhatishgarh

Prahlad Chandra Agrawal, Shiv Chandraker, Prarabdha Agrawal

Abstract


Background: HIV,HBV,& HCV is preventable transmitted disease, can accurately & correctly analyse by Elisa ,a superior method than rapid screening  of blood units & its components.

Aim: To see the positive results shown by Elisa method is positive by rapid or not. By this the superiority of method can be decided for screening of blood units in blood bank.

Method: Out of 3650  blood units a sample of 50 elisa reactives were retested by Rapid method & results are analysed & compared.

Only the higher Optical density gives positive result by rapid. Lower Optical density were not detected by rapid method, carry the risk of transmission to recipient.

Result: The result of Elisa is not parallel to rapid test. Many samples reactive by Elisa were not detected positive by rapid due to low OD.

Conclusion: Study shown that Rapid testing of samples for HIV, HBV& HCV are inferior to Elisa, Which is a gold standard for screening of blood donors in blood banks. 

 

DOI: 10.21276/AABS.1450

 


Keywords


Transmitted transfusion Diseases, Hepatitis C virus, Prevalence, Blood units

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References


Transfusion medicine. Technical manual, 2 nd ed. WHO; 2003. p. 151.

Aach RD, Szmuness W, Mosley JW, Hollinger FB, Kahn RA, Stevens CE, et al. Serum alanine aminotransferase of donors in relation to the risk of non-A,non-B hepatitis in recipients: the transfusion-transmitted viruses study. N Engl J Med. 1981; 304(17):989-94. PubMed | Google Scholar

Niederhauser C, Schneider P, Fopp M, Ruefer A, Levy G. Incidence of viral markers and evaluation of the estimated risk in the Swiss blood donor population from 1996 to 2003. Euro Surveill. 2005; 10(2):14-6. PubMed | Google Scholar

El-Sherif AM, Abou-Shady MA, Al-Hiatmy MA, Al-Bahrawy AM, Motawea EA. Screening for hepatitis B virus infection in Egyptian blood donors negative for hepatitis B surface antigen. Hepatol Int. 2007; 1(4):469-70. PubMed | Google Scholar

Busch MP, Glynn SA, Stramer SL, Strong DM, Caglioti S, Wright DJ, et al. A new strategy for estimating risks of transfusion-transmitted viral infections based on rates of detection of recently infected donors. Transfusion. 2005;45(2):254-64. PubMed | Google Scholar

McClelland DBL. Handbook of transfusion medicine : blood transfusion services of the United Kingdom. 2nd ed ed London: HMSO.1996. Google Scholar

Basavaraju SV, Mwangi J, Nyamongo J, Zeh C, Kimani D, Shiraishi RW, et al. Reduced risk of transfusion-transmitted HIV in Kenya through centrally co-ordinated blood centres, stringent donor selection and effective p24 antigen-HIV antibody screening. Vox Sang. 2010; 99(3):212-9. PubMed | Google Scholar

Rahman M, Khan SA, Lodhi Y. Unconfirmed reactive screening tests and their impact on donor management. Pak J Med Sci. 2008; 24(4):517-19. PubMed | Google Scholar

Torane VP, Shastri JS. Comparison of ELISA and rapid screening tests for the diagnosis of HIV, Hepatitis B and Hepatitis C among healthy blood donors in a tertiary care hospital in Mumbai. Indian J Med Microbiol. 2008; 26(3):284-5. PubMed | Google Scholar

Lathamani K., Bhaktha G, Nayak S.,Kotigadde S. Prevalence of HIV, HCV,HBV and Syphilis in Blood donors among the Dakshina Kannada District, India Int.J.Curr.Microbiol.App.Sci (2013) 2(10):249-252

Glynn SA, Kleinman SH, Wright DJ, Bush MP. For the NHLBI retrovirus epidemiology donor study: International application of incidence rate/ window period model. Transfusion 2002; 42: 966-72

Shah N, Shah J.M. , Jhaveri P, Patel K, Shah C.K., Shah N.R. Sero prevalence of

HBV, HCV, HIV and syphilis among blood donors at a tertiary Care Teaching Hospital in Western India GUJARAT MEDICAL JOURNAL ,2013 ; 68: 35 39

Sharma RR, Cheema R, Vajpayee M, Rao U, Kumar S, Marwaha N, et al. Prevalance

of markers of transfusion transmissible diseases in voluntary and replacement blood donors. The national medical journal of India 2004; 171: 19 -21.

Paramjit K, Basu S. Transfusion transmitted infections: Existing and emerging pathogens. Journal of post graduate medicine 2005;51:146–51

World health organization South-East Asia Regional Office. Prevention of hepatitis B

in India-an overview. Available from: whqlibdoc.who.int/searo/2002/SEA_Hepat .-5.pdf, accessed on June 10, 2014

Chandrasekaran S, Palaniappan N, Krishnan V, Mohan G, Chandrasekaran N. Relative prevalence of hepatitis B viral markers and hepatitis C virus antibodies (Anti HCV) in Madurai, South India Indian journal of medical sciences 2000;547:270-73

Khan MA, Rehaman A, Ashraf M, Ali A, Detta A. Prevalance of HPV, HCV and HIV in blood donors at Liaquatpur.Professional med j 2006;13:23-26.

Khan Z, Raziq F, Aslam N. Prevalance of HIV in blood doors in NWFP. J Postgradmed inst 2002;16:187-89.

Saha SK, Banik RK, Saha MR, Habibullah MM, Mahtab MA. Prevalance of transfusion transmitted infectio in healthy blood donors in sir Salimullah medical college Dhaka, Bangladesh. Euroasianjournals of hepato-gastroenterology,2011;1:68-70.

Saha SK, Banik RK, Saha MR, Habibullah MM, Mahtab MA. Prevalance of transfusion transmitted infectio in healthy blood donors in sir Salimullah medical college Dhaka, Bangladesh. Euroasian journals of hepato-gastroenterology, 2011;1:68-70.


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