Isolation & Characterization of Potential Microbe for Bio-remediating Heavy Metal from Mithi River

  • Pooja Inder Sevak Department of Biotechnology, University of Mumbai
  • Bhupendra K. Pushkar University of Mumbai
  • Anamika Singh University of Mumbai
Keywords: Isolation, characterization, bioremediation, heavy metal, pollutants

Abstract

Background:Heavy metals contamination is common problem in the current time in river water and other natural resources.Mithi River receives the different kind of industrial and domestic wastes, which also includes toxic heavy metal pollutants. Bioremediation of the heavy metal pollutants can be a promising option for their removal.Methods:Isolation and characterization of the heavy metal resistant microorganisms obtained from the Mithi River was performed using basic biochemical test and confirmed by 16S rDNA technique. SEM analysis, MIC and flocculation index were carried out to detect the efficiency of bacteria for bioremediation.Results:A significant population of heavy metal resistant microorganisms were found in Mithi River. Microorganisms of Mithi Riverstudied in the current research were gram negative as well as gram positive.Conclusion:Microorganisms having the potential of bioremediation of heavy metals were isolated successfully from Mithi River and their identity was confirmed. The potential of the microorganism for their capacity of bioremediation of heavy metals was also confirmed. 

Author Biographies

Pooja Inder Sevak, Department of Biotechnology, University of Mumbai
Department of Biotechnology, JRF
Bhupendra K. Pushkar, University of Mumbai
Department of Biotechnology, University of Mumbai, Kalina, Santacruz (E), Mumbai, 400098, Maharashtra, India.
Anamika Singh, University of Mumbai
Department of Biotechnology, University of Mumbai

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Published
2015-03-29
Issue
Vol 2 No 2 (2015)
Section
Original Article

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