Isolation & Characterization of Potential Microbe for Bio-remediating Heavy Metal from Mithi River

  • Pooja Inder Sevak Department of Biotechnology, University of Mumbai
  • Bhupendra K. Pushkar University of Mumbai
  • Anamika Singh University of Mumbai
Keywords: Isolation, characterization, bioremediation, heavy metal, pollutants


Background:Heavy metals contamination is common problem in the current time in river water and other natural resources.Mithi River receives the different kind of industrial and domestic wastes, which also includes toxic heavy metal pollutants. Bioremediation of the heavy metal pollutants can be a promising option for their removal.Methods:Isolation and characterization of the heavy metal resistant microorganisms obtained from the Mithi River was performed using basic biochemical test and confirmed by 16S rDNA technique. SEM analysis, MIC and flocculation index were carried out to detect the efficiency of bacteria for bioremediation.Results:A significant population of heavy metal resistant microorganisms were found in Mithi River. Microorganisms of Mithi Riverstudied in the current research were gram negative as well as gram positive.Conclusion:Microorganisms having the potential of bioremediation of heavy metals were isolated successfully from Mithi River and their identity was confirmed. The potential of the microorganism for their capacity of bioremediation of heavy metals was also confirmed. 

Author Biographies

Pooja Inder Sevak, Department of Biotechnology, University of Mumbai
Department of Biotechnology, JRF
Bhupendra K. Pushkar, University of Mumbai
Department of Biotechnology, University of Mumbai, Kalina, Santacruz (E), Mumbai, 400098, Maharashtra, India.
Anamika Singh, University of Mumbai
Department of Biotechnology, University of Mumbai


1. Munees Ahemad. Implication of Bacterial Resistance against Heavy Metals in Bioremediation: A REVIEW. ISSN: 0976-3104, 2012.
2. Ashok Kumar, B.S. Bisht, V.D. Joshi. Bioremediation potential of three acclimated bacteria with reference to heavy metal removal from waste. International Journal of Environment Sciences Volume 2, No 2, 2011.
3. J.P. Rupareliaa, S.P. Duttaguptab, A.K. Chatterjeec and S. Mukherjia. Potential of carbon nanomaterials for removal of heavy metalsfrom water. Volume 232, Issues 1–3, 30 November 2008, Pages 145–156.
4. MeghrajHookoom and DaneshwarPuchooa.Isolation and Identification of Heavy Metals Tolerant Bacteria from Industrial and Agricultural Areas in Mauritius. Current Research in Microbiology and Biotechnology, Vol. 1, No. 3 (2013): 119-123.
5. M.A. Hashim, Soumyadeep Mukhopadhyay, Jaya Narayan SahuandBhaskarSengupta. Remediation technologies for heavy metal contaminated groundwater. Journal of Environmental Management 92 (2011) 2355e2388.
6. RashmiVerma and PratimaDwivedi. Heavy metal water pollution- A case study. Recent Research in Science and Technology 2013, 5(5): 98-99.
7. Akshata Jain N, Udayashankara T. H andLokesh K. S.Review on Bioremediation of Heavy Metals withMicrobial Isolates and Amendments on Soil Residue. International Journal of Science and Research, 2012, ISSN (Online): 2319-7064.
8. Lars Järup. Hazards of heavy metal contamination. British Medical Bulletin 2003; 68: 167–182.
9. Reena Singh, NeetuGautam, Anurag Mishra, and Rajiv Gupta. Heavy metals and living systems: An overview. Indian J Pharmacol. 2011 May-Jun; 43(3): 246–253.
10. M. Pepi, M. Arsenic-resistant bacteria isolated from contaminated sediments of the Orbetello Lagoon, Italy, and their characterization. Journal of Applied Microbiology ISSN 1364-5072, 2007.

11. Shailendra Singh, Seung Hyun Kang, Ashok Mulchandani and Wilfred Chen. Bioremediation: environmental clean-up through pathway engineering. Current Opinion in Biotechnology, 19:437–444, 2008.

12. C. Garbisu and I. Alkorta. Basic concepts on heavy metal soil bioremediation. The European Journal of Mineral Processing and Environmental Protection, Vol.3, No.1, 1303-0868, 2003, pp. 58-66.

13. Shruti Murthy, Geetha Bali and S. K. Sarangi. Biosorption of Lead by Bacillus cereus Isolated from Industrial Effluents. British Biotechnology Journal 2(2): 73-84, 2012.
14. Hadis Ghodsi, MehranHoodaji, ArezooTahmourespour and Mohammad Mehdi Gheisari. Investigation of bioremediation of arsenic by bacteria isolated from contaminated soil. African Journal of Microbiology Research Vol. 5(32), pp. 5889-5895, 30 December, 2011.
15. MuneesAhemad and Abdul Malik. Bioaccumulation of Zinc Resistance Bacteria Isolated from Agricultural Soil Irrigated With Wastewater. Bacteriology Journal, ISSN 2153-0211/DOI:10.3923/bj. 2011.
16. M. H. Fulekar, Jaya Sharma &Akalpita Tendulkar. Bioremediation of heavy metals using biostimulationin laboratory bioreactor.EnvironMonit Assess 184:7299–7307.
17. JinHee PARK, Nanthi BOLAN1, Mallavarapu MEGHARAJ, Ravi NAIDUand Jae Woo CHUNG. Bacterial-Assisted Immobilization of Lead in Soils: Implications for Remediation. Pedologist (2011) 162-174.
18. Rajiv K. Sinha, DalsukhValani, Shanu Sinha, Shweta Singh & Sunil Herat. Bioremediation of Contaminated Sites: A Low-Cost Nature’s Biotechnology for Environmental Clean up by Versatile Microbes, Plants & Earthworms. In: Solid Waste Management and Environmental Remediation ISBN: 978-1-60741-761-3, 2009.
19. C. Edward Raja, Identification and Characterization of Heavy Metal Resistant Bacteria from Sewage. International Joint Symposium on Geo-disaster Prevention and Geo-environment in Asia JS-Fukuoka, 2009.
20. Report on Mithi River Water Pollution and Recommendations for its Control Submitted by Maharashtra pollution Control Board, 2004.
21. Current Status of Mithi River and Possible Solution a report by National Environmental Engineering Research Institute, 2011.
22. M. Saleem, H. Brim b, S. Hussain, M. Arshad, M.B. Leigh, Zia-ul-hassan. Perspectives on microbial cell surface display in bioremediation. Biotechnology Advances 26 (2008) 151–161.
23. Pandit R.J., Patel B, Kunjadia P.D and Nagee A. Isolation, characterization and molecular identification of heavy metal resistant bacteria from industrial effluents, Amala-khadi- Ankleshwar, Gujarat. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCES Volume 3, No 5. 2013.
24. Sannasi P., Kader J., Othman O. and Salmijah S. PHYSICAL GROWTH AND BIOMASS CHARACTERIZATION OF BACTERIAL CELLS EXPOSED TO Cd(II), Cr(VI), Cu(II), Ni(II), AND Pb(II). Journal of Environmental Research and Development Vol. 4 No. 1, July-September 2009.
Original Article