Assessment Of Peripheral Blood Smear Preparation Technique In Laboratories With High Sample Load

  • Sumanashree Mallappa Vardhman Mahavir Medical College and Safdarjung Hospital
  • Sachin Kolte VMMC & Safdarjung hospital
  • Nimisha Sharma VMMC & Safdarjung hospital
  • Indrani Dhawan VMMC & Safdarjung Hospital
Keywords: Peripheral Smear High Case Load Carry Over Of Cells

Abstract

Background: Examination of the blood film is an important part of the hematological evaluation. The reliability of the information obtained depends heavily on well-made and well-stained films that are systematically examined. Commonly used method of smear preparation in hematology the wedge method. In wedge method two slides are used one slide is placed on a flat surface with all necessary conditions maintained and the other slide is used as a spreader slide with is used to spread the blood film producing a good tongue shaped smear.In laboratory settings with high case load, same spreader is used to produce blood smears of multiple patients. Methods: The study was  designed to know if there is any carry over of cells if the same spreader is used to make blood smears of multiple patients in laboratories having high patient load. Same spreader was used to make multiple smears from pancytopenia sample after using it initially to make smears from samples of leukemia and neutrophilic leucocytosis. Result: Slides were examined and results were tabulated, which showed that there was a definite carry over of cells from one smear to another if the same spreader is used consequetively for multiple patients Conclusion: Caution need to be used in laboratories with high case load where multiple smears need to be prepared and time constraint is a limiting factor. Use of advanced coulters can help in correlation of cells counts and typing instead of only depending on smears for cells counts and reporting. Collecting clinical details of the patient is an important step which is indispensable.  DOI: 10.21276/AABS.1655

Author Biographies

Sumanashree Mallappa, Vardhman Mahavir Medical College and Safdarjung Hospital
Pathology senior resident
Sachin Kolte, VMMC & Safdarjung hospital
Associate professor in pathology
Nimisha Sharma, VMMC & Safdarjung hospital
Senior resident in pathology
Indrani Dhawan, VMMC & Safdarjung Hospital
HOD  in pathology

References

1. Vajpayee N, Graham SS, Sylva Bem. Basic Examination of Blood and Bone Marrow. In: McPherson RA, Pincus MR. Henry′s Clinical Diagnosis and Management by Laboratory Methods. 21st ed. Philadelphia: Elsevier Saunders; 2007.

2. Bain BJ. Diagnosis from the blood Smear. N Engl J Med. 2005;353:498–507

3. Levine MS, Levine DS. Automatic blood film preparation method.1998

4. Ryan DH. Automated analysis of blood cells. In: Hoffman R, Benz EJ, Shattil SJ, Furie B, Cohen HJ, Silberstein LE, editors. Hematology: basic principles and practice. 2nd ed. New York: Churchill Livingstone; 1995. pp. 2223–2235.

5. Buttarello M, Plebani M. Automated blood cell counts: state of the art. Am J Clin Pathol. 2008;130(1):104–116

6. Barnes PW, McFadden SL, Machin SJ, Simson E, International Consensus Group for Hematology The international consensus group for hematology review: suggested criteria for action following automated CBC and WBC differential analysis. Lab Hematol. 2005;11(2):83–90
Published
2017-10-14
Section
Original Article