Tender Coconut (Cocos nucifera L.) husk anaerobic leachate as a potent antifungal and antibacterial agent

  • Praveen Krishnakumar Immunology & Toxicology Research Lab, Dept. of Zoology, Christ College, Irinjalakuda. Kerala, India.
  • Treesamol Antony
Keywords: Phenolic compounds, antibacterial activity, antifungal activity, lyophilized leachate, raw leachate


Background: Leaching of tender coconut husk in anaerobic condition may leads to the production of a wide range of secondary metabolites including, alkaloids, terpenoids and phenolic compounds. In this study we have evaluated the antibacterial and antifungal potential of tender coconut husk leachate against Escherichia coli, Lactobacillus plantarum, Staphylococcus aureus, Klebsiella pneumoniae, Penicillium sps, Aspergillus niger and Mucor indicus.Methods: Leachate was treated separately as unaltered raw leachate and lyophilized leachate. Qualitative phytochemical analysis of the tender coconut husk leachate was carried out using standard procedures. There are differences in the constituents present with raw and lyophilized leachate. Antibacterial activity were determined using disc diffusion method and also by calculating MIC and MBC. Antifungal activity was determined using poison plate method.Result: Anaerobic leaching leads to the extraction of potent secondary metabolites which possess antbacterial and antifungal activities. Both the lyophilized and air dried raw leachate was effective in preventing the growth of gram negative bacteria (E.coli and K.pneumoniae) whereas the gram positive bacteria ( S.aureus and L.plantarum) were least affected. In higher concentrations leachate exhibited similar inhibitory activity as that of positive control used. We could also observe a concentration dependent growth inhibition in majority of the treatments.Conclusion: From this study we could conclude that the anaerobic leachate of tender coconut husk exhibits antimicrobial activity against different pathogenic organisms. In all the trials the raw and lyophilized leachate showed very prominent control over both the bacteria and fungus studied. Further studies like specific characterization of potent molecules from the leachate and their action on multidrug resistant microorganisms are necessary to develop effective and safe antimicrobial products. 

Author Biography

Praveen Krishnakumar, Immunology & Toxicology Research Lab, Dept. of Zoology, Christ College, Irinjalakuda. Kerala, India.


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Original Article