Quality of Cold Centrifuge Affect on PCR Results

  • Usha .
  • Shailja Singh "Banaras Hindu University"
  • Niladri .
  • Ranjan Singh Rana
  • Kavyanjali Sharma
  • Praveen Kumar
Keywords: HLAB-27, DNA Extraction, PCR.


See Full text DNA extraction is an important step for PCR test. We are doing DNA extraction by salt-out and Phenol Chloroform method using two cold centrifuge instruments, one PSN Instrumentation Pvt. Ltd, Delhi Company and the other one Plasto Craft industries (P) Ltd. Mumbai. We performed HLAB-27 typing by conventional allele-specific PCR and the kit of BAG Company supplied by M/S Shiva Scientific. Using these two methods, we found that DNA extracted in PSN cold centrifuge gave false positive band in most cases while DNA extracted by Plasto Craft cold centrifuge did not give any false positive result in HLAB-27. Up till now it was considered that type and amount of sample, its collection, pipettting, quality of distilled water, contamination and quality of reagents, thermal cycle programme and gel electrophoresis affect PCR results. However our study suggest that quality of  cold centrifuge also affect the quantity of DNA, PCR results and give false positive results and hence cold centrifuge should be of good quality and calibrated. 

Author Biography

Shailja Singh, "Banaras Hindu University"
Shailja Singh is a Ph.D. Student in the UGC Advanced Immunodiagnostic Training & Research Centre, Department of Pathology, Institute of Medical Sciences, Banaras Hindu University, Varanasi. She has published research paper in various journals and also presented various paper in national and international conferences.


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