Prevalence of Non-albicans candida and its drug susceptibility pattern isolated from a tertiary care hospital, Karnataka
Keywords:
Candida, candidiasis, chromogenic media, azole resistance.
Abstract
Background: Candida infections have increased over the past few decades. Candida species are opportunistic yeast infections which are also common commensal of human gastro-intestinal tract. It causes spectrum of diseases extending from Mucocutaneous infections to systemic infection with dissemination. The increased incidence of life threatening infections in immunocompromised hosts and the emergence of non albicans candida (NAC) have made the treatment options difficult. Over to it, rampant use of azoles as prophylaxis has led to the emergence of azole resistant strains which are of great concern for clinical and therapeutic aspects. The present study was taken up with the aim to isolate and identify the candida species from clinical samples using a chromogenic media and to derive anti-fungal susceptibility pattern.Methods: Total 484 samples was processed using Hichrome agar media and idenfied using standard identification methods up to species level. Antifungal susceptibility tests were performed according to CLSI guidelines for Fluconazole, Voriconazole, Ketoconazole and Itraconazole.Results: Total 484 clinical specimens was collected from all the department. Out of which 176 candida species was isolated. Predominant isolate was C. tropicalis followed by C. guilliermondi and C. parapsilosis. Hichrome agar showed good sensitivity and specificity. Voriconazole (77.5%) showed good sensitivity over Fluconazole (41.4%) sensitivity.Conclusion: present study highlights there is need to identify NAC and give sensitivity pattern.References
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2. Kumari V, Banerjee T, Kumar P, Pandey S, Tilak R. Emergence of non- albicans Candida among candidal vulvovaginitis cases and study of their potential virulence factors, from a tertiary care center, North India. Ind J Path Microbiol 2012; 56:144-7.
3. Pfaller MA, Houstan A, Coffman S. Application of CHROM agar Candida for rapid screening of clinical specimens for Candida albicans, Can-dida tropicalis, Candida krusei and Candida glabrata. J Clin Microbiol 1996; 34:58-61.
4. Chander J. Text book of Medical Mycology. 3rd Ed Chapter 20, Mehta Offset Pvt Ltd, New Delhi 2010; pg 266-90.
5. Nadiger SD, Chunchanur SK, Halesh LH, Yasmeen K, Chandrashekar MR, Patil BS. Sig-nificance of isolation and drug susceptibility testing of non- Candida albicans species causing oropharyngeal candidiasis in HIV patients. J Clin Microbiol 2008; 39:492-5.
6. Trofa D, Gacser A, Nosanchuk JD. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol rev 2008; 21: 606-25.
7. Anupriya W et al, AIDS-related opportunistic mycoses seen in a tertiary care hospital in North. India Journal of Medical Microbiology 2007; 56:1101–6.
8. Baradkar VP, Mathur M, Kumar S. Hicrome Candida agar for identification of Candida spe-cies. Ind J Patho and Microbiol 2010; 53: 93-95.
9. Walmsley S, King S, McGeer A, Yanna Ye, Richardson S. Oropharyngeal Candidiasis in Patients with Human Immunodeficiency Virus: Correlation of Clinical Outcome with In Vitro Resistance, Serum Azole Levels, and Immuno-suppression, CID 2001;32: 1554-61.
10. Francisca I, Okungbowa, Chowdhury R, Pal K. Susceptibility of Candida isolates to Amphote-ricin B and Imidazole: effect of CCCP on Im-idazole activity. Int J Biomed and health Sciences 2009; 5: 109-113.
Published
2015-01-29
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