Dignostic Utility of HBME1 to Differentiate Between Reactive Mesothelial Cells and Adenocarcinoma Cells in Body Fluids

  • Charusheela Gore Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, Maharashtra, India
  • Karan Patel Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, Maharashtra, India
  • Rupali R Bavikar Associate professor
  • Ashly . Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, Maharashtra, India
Keywords: Adenocarcinoma, Reactive mesothelial cells, HBME1

Abstract

Background : Reactive mesothelial cells  show overlapping features with malignant cells.To differentiate between the two Immunocytochemistry  (ICC) can be used.HBME1 is the antigen present on mesothelial cells.We have tried to evaluate  the efficacy of HBME1 to differentiate between reactive mesothelial cells and adenocarcinoma cells. Methods : Total 60 cases with clinical dilemma or suspicious on conventional cytology were selected .Cell block was prepared and staining with monoclonal antibody HBME1 was done.Staining pattern was classified as cytoplasmic ,membranous or combined. Result : Out of 42 ,28 cases showed negative staining ,8 cases showed cytoplasmic positivity and 4 cases showed combined positivity.All the benign cases showed membranous potivity. Conclusion:The staining pattern of HBME1 can be used to differentiate between reactive  mesothelial cells and adenocarcinoma cells.

Author Biographies

Charusheela Gore, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, Maharashtra, India
Dept. of Pathology
Karan Patel, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, Maharashtra, India
Dept. of Pathology
Ashly ., Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, Maharashtra, India
Dept. of Pathology

References

1 Lozano MD, Panizo A, Toledo GR, Sola JJ, Pardo-Minda J. Immunocytochemistry in the differential diagnosis of serous effusions. A comparative evaluation of eight monoclonal antibodies in Papanicolaou stained smears. Cancer Cytopathol 2001;93:68-72.
2. Fetsch PA, Abati A. Immunocytochemistry in effusion cytology. Cancer Cytopathol 2001;93:293-308.
3.Sayed DM, el-Attar MM, Hussein AA. Evaluation of flow cytometric immunophenotyping and DNA analysis for detection of malignant cells in serosal cavity fluids. Diagn Cytopathol 2009;37:498-504.
4. Krausz T, Barker F. Metastatic disease and lymphomas. In: Gray W, McKee GT, editors. Diagnostic Cytopathology. 2nd ed. London: Churchill- Livingstone; 2003. p. 135-200.
5 Murugan P, Siddaraju N, Habeebullah S, Basu D. Immunohistochemical distinction between mesothelial and adenocarcinoma cells in serous effusions: A combination panel-based approach with a brief review of the literature. Indian J Pathol Microbiol 2009;52:175-81.
6. Henzi T, Blum WV, Pfefferli M, Kawecki TJ, Salicio V, Schwaller B. SV40-induced expression of calretinin protects mesothelial cells from asbestos cytotoxicity and may be a key factor contributing to mesothelioma pathogenesis. Am J Pathol 2009;174:2324-36.
7. Wu GP, Zhang SS, Fang CQ, Liu SL, Wang EH. Immunocytochemical panel for distinguishing carcinoma cells from reactive mesothelial cells in pleural effusions. Cytopathology 2008;19:212-7.
8. Mocanu L, Cîmpean AM, Raica M. Value of antimesothelioma HBME-1 in the diagnosis of inflammatory and malignant pleural effusions. Rom J Morphol Embryol 2006;47:351-5.
9. Marchevsky AM, Wick MR. Evidence-based guidelines for the utilization of immunostains in diagnostic pathology: Pulmonary adenocarcinoma versus mesothelioma. Appl Immunohistochem Mol Morphol 2007;15:140-4.
10. Ascoli V, Carnovale-Scalzo C, Taccogna S, Nardi F. Utility of HBME- 1 immunostaining in serous effusions. Cytopathology 1997;8:328-35.
11. Rehmani A, Dehgani MZ, Afshar NM, Heidarian H, Tahririan R. HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid. Indian J Pathol Microbiol. 2011;54:460-3.
12. Szczepulska-Wójcik E, Langfort R, Roszkowski-Sliz K. A comparative evaluation of immunohistochemical markers for the differential diagnosis between malignant mesothelioma, non-small cell carcinoma involving the pleura, and benign reactive mesothelial cell proliferation. Pnumonol Alergol Pol 2007;75:57-69.
13. Politi E, Kandaraki C, Apostolopoulou C, Kyritsi T, Koutselini H. Immunocytochemical panel for distinguishing between carcinoma and reactive mesothelial cells in body cavity fluids. Diagn Cytopathol 2005;32:151-5.
1 Lozano MD, Panizo A, Toledo GR, Sola JJ, Pardo-Minda J. Immunocytochemistry in the differential diagnosis of serous effusions. A comparative evaluation of eight monoclonal antibodies in Papanicolaou stained smears. Cancer Cytopathol 2001;93:68-72.
2. Fetsch PA, Abati A. Immunocytochemistry in effusion cytology. Cancer Cytopathol 2001;93:293-308.
3.Sayed DM, el-Attar MM, Hussein AA. Evaluation of flow cytometric immunophenotyping and DNA analysis for detection of malignant cells in serosal cavity fluids. Diagn Cytopathol 2009;37:498-504.
4. Krausz T, Barker F. Metastatic disease and lymphomas. In: Gray W, McKee GT, editors. Diagnostic Cytopathology. 2nd ed. London: Churchill- Livingstone; 2003. p. 135-200.
5 Murugan P, Siddaraju N, Habeebullah S, Basu D. Immunohistochemical distinction between mesothelial and adenocarcinoma cells in serous effusions: A combination panel-based approach with a brief review of the literature. Indian J Pathol Microbiol 2009;52:175-81.
6. Henzi T, Blum WV, Pfefferli M, Kawecki TJ, Salicio V, Schwaller B. SV40-induced expression of calretinin protects mesothelial cells from asbestos cytotoxicity and may be a key factor contributing to mesothelioma pathogenesis. Am J Pathol 2009;174:2324-36.
7. Wu GP, Zhang SS, Fang CQ, Liu SL, Wang EH. Immunocytochemical panel for distinguishing carcinoma cells from reactive mesothelial cells in pleural effusions. Cytopathology 2008;19:212-7.
8. Mocanu L, Cîmpean AM, Raica M. Value of antimesothelioma HBME-1 in the diagnosis of inflammatory and malignant pleural effusions. Rom J Morphol Embryol 2006;47:351-5.
9. Marchevsky AM, Wick MR. Evidence-based guidelines for the utilization of immunostains in diagnostic pathology: Pulmonary adenocarcinoma versus mesothelioma. Appl Immunohistochem Mol Morphol 2007;15:140-4.
10. Ascoli V, Carnovale-Scalzo C, Taccogna S, Nardi F. Utility of HBME- 1 immunostaining in serous effusions. Cytopathology 1997;8:328-35.
11. Rehmani A, Dehgani MZ, Afshar NM, Heidarian H, Tahririan R. HBME-1 immunostaining in reactive mesothelial versus metastatic adenocarcinoma cells in serous fluid. Indian J Pathol Microbiol. 2011;54:460-3.
12. Szczepulska-Wójcik E, Langfort R, Roszkowski-Sliz K. A comparative evaluation of immunohistochemical markers for the differential diagnosis between malignant mesothelioma, non-small cell carcinoma involving the pleura, and benign reactive mesothelial cell proliferation. Pnumonol Alergol Pol 2007;75:57-69.
13. Politi E, Kandaraki C, Apostolopoulou C, Kyritsi T, Koutselini H. Immunocytochemical panel for distinguishing between carcinoma and reactive mesothelial cells in body cavity fluids. Diagn Cytopathol 2005;32:151-5.
Published
2018-07-20
Section
Original Article