Evaluation of Platelet counts and its Indices in Various Clinical Conditions using Automated Hematology Analyzer in a Tertiary care Hospital

  • Niraimathi Manickam Pondicherry Institute of Medical Sciences, Puducherry
  • Renu G'boy Varghese Pondicherry Institute of Medical Sciences, Puducherry, India
  • Anand Mohanraj Pondicherry Institute of Medical Sciences, Puducherry, India
Keywords: Platelet count, Mean Platelet Volume, Plateletcrit, Platelet Distribution Width


Background and Aim: With wide use of Automated hematology analyzers, precise information on platelet indices viz. Mean Platelet Volume (MPV), Plateletcrit (PCT) and Platelet Distribution Width (PDW) are easily estimated. The aim was to correlate platelet indices in various clinical conditions. Materials and methods: It was a retrospective record based study for one month.  Samples (n=501) were processed in ABX Pentra DF120 analyzer. To detect differences among various clinical conditions One-way ANNOVA test was used. Results: Of 501 samples, 386 (77%) showed normal counts, 57 (11.4%) with thrombocytopenia and 58 (11.6%) with thrombocytosis. Significant decrease in platelet count, PCT with increase in MPV, PDW was seen in cases of ITP and hepatic encephalopathy. Patients with bronchiectasis, fever and alcoholic liver disease showed increased MPV, PDW while tuberculosis and sepsis cases showed low levels with normal platelet counts. Conclusion: Platelet indices varied significantly in different clinical conditions. It can be used as an additional marker of platelet activation and inflammation.


1. Shah AR, Chaudhari SN, Shah MH. Role of Platelet parameters in diagnosing various clinical conditions. Natl J Med Res. 2013;3:162- 5.
2. Botma J, Mogongoa LF, Jaftha AD, Janse van Rensburg W. Reference ranges for platelet indices using Sysmex XE-2100 blood analyser. Medical technology SA 2012;26(2):17-21.
3. Suvak B, Torun S, Yildiz H, Sayilir A, Yesil Y, Tas A et al. Mean platelet volume is a useful indicator of systemic inflammation in cirrhotic patients with ascitic fluid infection. Annals of Hepatology. 2013;12(2):294-300.
4. Cabar FR, Fettback PB, Pereira P, Zugaib M. Serum markers in the diagnosis of tubal pregnancy. Clinics. 2008;63:701-8.
5. Artunc UB, Pala HG, Calik E, Oruc KS. Can mean platelet volume and platelet distrubition width be possible markers or ectopic pregnancy and tubal rupture? (MPV and PDW in ectopic pregnancy). Pak J Med Sci. 2014;30(2):352-5.
6. Nergiz AS, Altınkaya S, Kü.ük M, Demircan SS, Yüksel H. The association between platelet indices and recurrent pregnanacy loss.Gynecol Obstet Reprod Med. 2014;20:146-9.
7. Van der Lelie J, Von dem Borne AEG. Increased mean plate¬let volume in septicaemia. J Clin Pathol. 1983;36:693-6.
8. Karpatkin S. Heterogeneity of human platelets. I. Metabolic and kinetic evidence suggestive of young and old platelets. J Clin Invest. 1969;48:1073-82.
9. Paulus JM. Platelet size in man. Blood. 1975;46:321-36.
10. Karpatkin S, Garg SK, Siskind GW. Autoimmune thrombocytopenic purpura and the compensated thrombolytic state. Am J Med. 1971;51:1-4.
11. Bain BJ, Bates I. Basic haematological techniques. In: Lewis SM, Bain BJ, Bates I, editors. Dacie and Lewis practical haematology. 9th edn. Edinburgh: Churchill Livingstone 2001.
12. Öztürk ZA, Dag MS, Kuyumcu ME, Cam H, Yesil Y, Yilmaz N et al. Could platelet indices be new biomarkers for inflammatory bowel diseases? Eur Rev Med Pharmacol Sci. 2013;17:334-41.
13. Jackson SR, Carter JM. Platelet volume: laboratory measurement and clinical application. Blood Rev. 1993;7(2):104-13.
14. Chandrashekar V. Plateletcrit as screening tool for detection of platelet quantitative disorders. J Hematol. 2013;2(1):22-6.
15. Guclu E, Durmaz Y, Karabay O. Effect of severe sepsis on platelet count and their indices. Afr Health Sci. 2013;13(2): 333-8.
16. Peck-Radoslavljevic M. Hypersplenism. Eur J Gastroenterol Hepatol. 2001;13: 317-23.
17. Paniagua PA, Flores AG. Thrombocytopenia: risk factor for encephalopathy in a patient with liver failure. Med Int Mex. 2010;26(6):561-7. 18. Hu Y, Lou Y, Chen Y, Mao W. Evaluation of mean platelet volume in patients with hepatitis B virus infection. Int J Clin Exp Med. 2014;7(11):4207-13.
19. Koc S, Eyibilen A, Erdogan AS. Mean platelet volume as inflammatory marker in
sinusitis. Eur J Gen Med. 2011;8(4):314-7.
20. Gasparyan AY, Ayvazyan L, Mikhailidis DP, Kitas GD. Mean Platelet Volume: A Link
Between Thrombosis and Inflammation? Curr Pharm Des. 2011;17:47-58.
21. Thompson CB, Jakubowski JA, Quinn PG, Deykin D, Valeri CR. Platelet size as a
determinant of platelet function. J Lab Clin Med. 1983;101:205-13.
22. Uysal P, Tuncel T, Erge DO, Hocaoglu AB, Karaman O, Uzuner N. Does Mean Platelet Volume in Children with Bronchiectasis Predict Exacerbations? International Journal of Hematology and Oncology. 2014;24:54-9.
23. Saxena D, Tandon M, Gedam B S. Role of Mean platelet volume in diagnosis of acute appendicitis. IJBR. 2015; 6(04):235-7.
24. Laffi G, Marra F, Gresele P, Romagnoli P, Palermo A, Bartolini O et al. Evidence for a
storage pool defect in platelets from cirrhotic patients with defective aggregation.
Gasteroenterology. 1992;103(2): 641-6.
25. Ogawa T, Uchida H, Kusumoto Y, Mori Y, Yamamura Y, Hamada S. Increase in tumor necrosis factor alpha-and interleukin-6-secreting cells in peripheral blood mononuclear cells from subjects infected with Mycobacterium tuberculosis. Infect Immun. 1991;59(9):3021-25.
26. Baynes RD, Bothwell TH, Flax H, McDonald TP, Atkinson P, Chetty N et al. Reactive thrombocytosis in pulmonary tuberculosis. J Clin Pathol. 1987;40(6):676-9.
27. Zampieri FG, Ranzani OT, Sabatoski V, Possolo de souza H, Barbeiro H, Cruz da Neto LM et al. An increase in mean platelet volume after admission is associated with higher mortality in critically ill patients. Ann of Intensive Care 2014;4:20-8.
Original Article

Most read articles by the same author(s)