A Study of Storage Related Changes and Effect of Refrigeration on Hematological Parameters and Blood Cell Morphology in EDTA Anticoagulated Blood
Keywords:
Storage artifacts, Blood cell count, EDTA, blood cell morphology
Abstract
Introduction: Delayed sample analysis result in changes in measured parameters and morphology of cells, leading to erroneous reports of complete blood counts and peripheral smear reporting. The present study was aimed at evaluating the changes in blood cell counts and morphology on storage, particularly in the local setting and temperature of a tropical country. Materials & Methods: 190 patients referred to the laboratory for complete blood counts and peripheral smear examinations were included in the study. Blood counts and peripheral smear examination was done at 0, 2, 6, and 24 hours. The 6 and 24 hour samples were further studied under three different temperatures, refrigerated, ambient room temperature and at 370C. Statistical difference between values of studied parameter at different time interval was tested using paired t-test. P < 0.05 was considered for statistical significance. Results: There was no significant difference between 0 hour& 2 hour samples in any of the parameters. However a significant change was noted in the platelet count between 6 hour refrigerated and 6 hour un-refrigerated. All the red cell parameters and platelet counts and cell morphology were significantly altered at 24 hours in refrigerated and unrefrigerated samples. Conclusions: Storage of EDTA blood causes changes in the counts and morphology of the cells. Within 2 hours no changes are expected, however if there is an anticipated delay beyond 2 hours, it is worthwhile to refrigerate the samples up to 6 hours, especially when platelet counts are desired.References
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15. Baca ME, Gulati G, Kocher W, Schwarting R. Effects of storage of blood at room temperature on hematologic parameters measured on Sysmex XE-2100. Lab Med2006; 37:28–36
2. Antwi-Baffour S, Quao E, Kyeremeh R, Mahmood SA. Prolong Storage of Blood in EDTA Has an Effect on the Morphology and Osmotic Fragility of Erythrocytes. Int J Biomed Sci Engg. 2013; 1:20-3.
3. Turhan T, S, Yucel C, Koca Y. Effects of storage conditions on complete blood cell count parameters. Turk J Biochem 2011;36;165-74.
4. Gulati GL. Hyland LJ, Kocher W, Schwarting R. Changes in automated com-plete blood cell count and differential leukocyte count results induced complete blood cell count and differential leukocyte count results induced by storage of blood at room temperature. Arch Pathol Lab Med 2002;126:336-42
5. Mane M, Kiran CM, Lakshmi TM, Reddy EP. Storage and Stability of Blood Samples – Changes in The Haematological Values. J Pharm Biomed Sci 2014;04:685-7.
6. McVicker W, Segalla R, Favaloro E, Luu V, Vanniasinkam T. Determining the stability of complete blood count parameters in stored blood samples using the SYSMEX XE-5000 automated haematology analyser. Int J Lab Hematol. 2015;37:705-14
7. Cohle SD, Saleem A, Makkaoui DE. Effects of storage of blood on stability of hematologic parameters. Am J Clin Pathol 1981;76:67–9.
8. Zini, G. International Council for Standardization in haematology (ICSH) (), Stability of complete blood count parameters with storage: toward defined specifications for different diagnostic applications. Int Jnl Lab Hem 2014;36:111-3.
9. Houven B. Blood film preparation and staining procedures. Lab Hematol 2000;6:1–7.
10. Vives-Corrons J-L, Briggs C, Simon-Lopez R, Albarede S, de la Salle B, Flegar-Meatrii Z et al, Effect of EDTA anticoagulated whole blood storage on cell morphology examination. A need for standardization. Int Jnl Lab Hem 2014; 36: 222–6.
11. Cora MC, King D, Betz LJ, Wilson R & Travlos GS. Artefactual changes in Sprague-dawley rat hematologic parameters after storage of samples C. J Am Assoc Lab Anim Sci 2012; 51: 611-21.
12. Daves M, Zagler EM, Cemin R, Flora F, Joos A, Platzgummer S et al Blood Sample stability for complete blood cell count using the Sysmex XN haematological analyser. Transfus 2015; 13: 576-82
13. Imeri F, Herklotz R, Risch L, Arbetsleitner C, Zerlauth M, Risch GM, et al. Stability of hematological analytes depends on the hematology analyser used: a stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sys-mex XE 2100. Clin Chim Acta 2008; 397:68–71.
14. Buoro S, Mecca T, Seghezzi M, Manenti B, Cerutti L, Dominoni P, et al. As-sessment of blood sample stability for complete blood count using the Sysmex XN-9000 and Mindray BC-6800 analyzers. Rev Bras Hematol Hemoter 2016; 38:225–39.
15. Baca ME, Gulati G, Kocher W, Schwarting R. Effects of storage of blood at room temperature on hematologic parameters measured on Sysmex XE-2100. Lab Med2006; 37:28–36
Published
2019-05-30
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