Evaluation of Epithelial Mesenchymal Transition Markers E-Cadherin and Vimentin in Carcinoma Cervix
Keywords:
Cervical carcinoma, Epithelial Mesenchymal Transition, Squamous Cell Carcinoma, Immunohistochemistry, E-Cadherin, Vimentin
Abstract
Background: Cervical cancer is the second most common in developing areas. Epithelial to mesenchymal transformation, one of the critical elements in invasion, progression and metastasis of tumour. This study highlights the expression of epithelial mesenchymal markers E-cadherin and vimentin in carcinoma cervix and whether there is any association of expression of these markers with grade of cervical cancer. Objectives: To study the expression of epithelial mesenchymal transition (EMT) markers E-cadherin and vimentin in cervical cancer. To correlate the immunohistochemical expression of these markers with grade of cervical cancer. Methods: 30 cases (n=30) of carcinoma cervix & 30 controls (n=30) of non specific cervicitis diagnosed on H&E were included in this study. H&E stained sections was examined for histological type and grade. Immunohistochemistry for E-Cadherin and Vimentin was performed in all these cases. Result: Immunohistochemical expression of epithelial marker E-cadherin and of mesenchymal marker vimentin was correlated with the grade of cervical carcinoma. The expression of E-cadherin is reduced and expression of vimentin is increased with increasing grades of carcinoma cervix. Conclusion: The expression of these EMT markers can be used as a prognostic marker in cervical cancer that are in high risk of progression.References
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2. LaMontagne DS, Barge S, Le NT, Mugisha E, Penny ME, Gandhi S et al. Human papillomavirus vaccine delivery strategies that achieved high coverage in low- and middle-income countries. Bull World Health Organ 2011;89:821-30.
3. Ferenczy A, France E. Persistent human papillomavirus infection and cervical neoplasia. Lancet Oncology 2002;3:11-16.
4. Walboomers J, Jacobs M, Manos MM, Bosch F, Kummer J, Shah K et al. Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol 1999;189:12–19.
5. Thiery JP, Sleeman JP. Complex networks orchestrate epithelial –mesenchymal transitions. Nat rev mol cell biol 2006;7:131-42.
6. Kalluri R. EMT: When epithelial cells decide to become mesenchymal like cells. J Clin Invest 2009;119:1417-9.
7. Yao D, Dai C, Peng S. Mechanism of the Mesenchymal–Epithelial Transition and Its Relationship with Metastatic Tumor Formation. Mol Cancer Res 2011;9:1608–20.
8. Korsching E, Packeisen J, Liedtke C, Hungermann D, Wulfing P, van Diest PJ et al. The origin of vimentin expression in invasive breast cancer: epithelial–mesenchymal transition, myoepithelial histogenesis or histogenesis from progenitor cells with bilinear differentiation potential?. J Pathol 2005;206:451–7.
9. Agnihotri P, Akhtar K, Siddiqui SA, Sherwani RK. Cervical Cancer Metastasis: Significance of E-Cadherin and Vimentin. Clin Res 2015;1:107.
10. Cheng Y, Zhou Y, Jiang W, Yang X, Zhu J, Feng D et al. Significance of E cadherin, β-catenin, and vimentin expression as postoperative prognosis indicators in cervical squamous cell carcinoma. Hum pathol 2012;43:121330.
11. Lee MY, Chou CY, Tang MJ, Shen MR. Epithelial-mesenchymal transition in cervical cancer: correlation with tumor progression, epidermal growth factor receptor overexpression, and snail upregulation. Clin Cancer Res 2008;14:4743-50.
Published
2020-07-07
Issue
Section
Original Article
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