Study of mast Cells in Appendectomy Specimen
Keywords:
Appendix, Mast cells, Toluidine blue
Abstract
Background: Mast cells play an important role in various inflammatory and immune reaction. Mast cells are constantly present in the appendices, and appendix remains one of the common surgical specimens received at pathology laboratory. Mast cells could be one of the important cell population responsible for nerve proliferation and hypertrophy in cases with clinically and histopathologically diagnosed appendicitis. Material and methods: The study was done on 100 surgically resected specimens of appendix received for routine histopathological evaluation at the Department of Pathology, Government Medical College, Bhavnagar, between June 2018 and May 2019. All appendices surgically resected as a therapeutic measure for clinically suspected appendicitis included. Cases of normal appendix and acute gangrenous appendicitis were excluded from the study. The appendices were assessed for their histological changes and density of mast cell infiltration. The mast cells were counted in 1% toluidine blue stained sections. Results: Out of 100 cases of surgically resected appendices, chronic appendicitis was found in 65% of cases and occurred frequently during the second and third decades of life. Highest mast cell count was seen in acute eosinophilic appendicitis and chronic appendicitis. No significant difference was observed in the mean mast cell counts among the different layers of the appendices studied. Conclusion: Mast cell counts were found to be highest in acute eosinophilic appendicitis and chronic appendicitis. Intermediate mast cell counts were seen in acute appendicitis and acute suppurative appendicitis. Mast cells appear to play roles in accentuation of inflammatory process and fibrosis.References
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18. Crow J, Howe S. Mast cell numbers in appendices with threadworm infestation. J Pathol 1988;154:347-351
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20. Sulochana Sonti. A study on the mast cells in appendicitis. Journal of Clinical and Diagnostic Research 2012 September 6:1276-1279.
2. Peters SP, Schleimer RP, Naclerio RM, Macglashan Jr DW, Togias AG, Proud D, Freeland HS, Fox C, Adkinson Jr NF, Lichtenstein LM. The pathophysiology of human mast cells: in vitro and in vivo function.Am Rev Respir Dis 1987;135:1196-1200.
3. Galli SJ, Tsai M. Mast cells: versatile regulators of inflammation, tissue remodeling, host defense and homeostasis. Journal of dermatological science. 2008 Jan 1;49(1):7-19.
4. Culling CFA, Allison RT, Barr WT: Cells and cell products of special interest – Miscellaneous cells. Cellular pathology technique, 1985, 4th Ed. Butterworth & Co., London; 459-464.
5. Aravindan KP. Eosinophils in acute appendicitis: possible significance. Indian Journal of Pathology and Microbiology. 1997 Oct 1;40:491-8.
6. Tsuji M, McMahon G, Reen D, Puri P. New insights into the pathogenesis of appendicitis based on immunocytochemical analysis of early immune response. Journal of pediatric surgery. 1990 Apr 1;25(4):449-52.
7. Rao KN, Brown MA. Mast cells: multifaceted immune cells with diverse roles in health and disease. Annals of the New York Academy of Sciences. 2008 Nov;1143(1):83-104.
8. Wang Y, Reen DJ, Puri P. Is a histologically normal appendix following emergency appendicectomy always normal?. The Lancet. 1996 Apr 20;347(9008):1076-9.
9. Williams GR. Presidential Address: a history of appendicitis. With anecdotes illustrating its importance. Annals of surgery. 1983 May;197(5):495.
10. Amber S, Mathai AM, Naik R, Pai MR, Kumar S, Prasad K. Neuronal hypertrophy and mast cells in histologically negative, clinically diagnosed acute appendicitis: a quantitative immunophenotypical analysis. Indian Journal of Gastroenterology. 2010 Mar 1;29(2):69-73.
11. Shrestha R, Ranabhat SR, Tiwari M. Histopathologic analysis of appendectomy specimens. Journal of pathology of Nepal. 2012;2(3):215-9.
12. Jerrold R, Turner. The Gastrointestinal Tract. In : Schmitt W, Kumar, et al. Robbins & Cotran Pathologic Basis of Disease, 8th ed ; 2010. p. 1344.
13. Kolur A, Patil AM, Agarwal V, Yendigiri S, Sajjanar BB. The Significance of mast cells and eosinophils counts in surgically resected appendix. Journal of interdisciplinary Histopathology. 2014;2(3):150-3.
14. Verma A, Shinde R.M, Ghatge R. M, Gupta S, Inamdar A.A and Sujata N (2016); A STUDY OF MAST CELLS IN SURGICALLY RESECTED APPENDIX. Int. J. of Adv. Res. 4 (11). 1547-1553] (ISSN 2320-5407).
15. Patel DM, Jitendra D, Joshi DD. Alpa A Shah. Medical Science. 2013 Jul;2(7).
16. Soma Datta.” Comparative study of mast cells in different pathological types of surgically resected appendix specimens ."IOSR Journal of Dental and Medical Sciences (IOSR-JDMS), vol. 17, no. 4, 2018 pp 52-57.
17. Sharma J,Chaudhary N,Bhargava S et al. Role of mast cells in appendicitis.Int J Health Sci Res.2017;7(5):37-41.
18. Crow J, Howe S. Mast cell numbers in appendices with threadworm infestation. J Pathol 1988;154:347-351
19. Mysorekar VV, Chanda S, Dandeka CP. Mast cells in surgically resected appendices. Indian J Pathol Microbiol 2006;49:229-233
20. Sulochana Sonti. A study on the mast cells in appendicitis. Journal of Clinical and Diagnostic Research 2012 September 6:1276-1279.
Published
2020-07-07
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