Significance Of Micro Vessel Density In Invasive Breast Carcinoma
Keywords:
Breast cancer, Micro vessel density, CD 34, Immunohistochemistry.
Abstract
Introduction: Breast cancer remains a leading cause of death in women of reproductive age group. Angiogenesis in breast carcinoma has been extensively studied and proven to play an important role is tumour outcome and patient prognosis. The prognostic relevance of angiogenesis expressed as microvessel density has been reported in various neoplasms.Aim: Our aim was to study the correlation of microvessel density in breast carcinomas.Materials and methods: Microvessel density quantification was done using immunohistochemistry technique with CD34 monoclonal antibody in a series of 55 cases of breast carcinoma. Microvessels were counted within a 200 X magnification field in the area of highest microvessel density and the results were correlated with tumour grade.Result: Microvessel density was highest in grade III breast tumours (132 ±18.7).Conclusion: In the present study, intra tumoral mean MVD was highest in grade III tumors suggesting that tumour angiogenesis correlates with tumour grade. However larger sample size would be required to decide cut-off values between different grades.DOI:10.21276/APALM.1436References
1. Bernard U, Patrick N, Michael C, Gerard Y P. Micro vessel Density as a Prognostic factor in women with Breast Cancer: A Systematic Review of Literature & Meta-analysis. Cancer Res 2004; 64: 2941-2955.
2. Kalyani R , Das S , Singh Bindra MS , Kumar HML. Cancer Profile in the Department of Pathology at Sri Devraj Urs Medical College ,Kolar : A ten years study. Indian Jr of Cancer 2010 ;47: 160-5.
3. Li HJ, Jing J, Zhao YB, Zhu JQ, Zhang SY, Shi ZD. Tumor Angiogenesis in Node Negative Breast Carcinoma. A I Zheng 2002; 21: 75-8.
4. McCarthy N J, Yang X, Linniola I R, Merino MJ, Hewitt SM, Parr AL et, al. Microvessel Density, Expression of Estrogen Receptor ,MIB 1, p53 & C erb 2 in Inflammatory Breast Cancer. Clin Cancer Res 2002; 8: 3857-62.
5. Da Silva BB, Lopes costa PV, dos Santos AR et al. Comparison of three Vascular Endothelial Markers in the Evaluation of Micro vessel Density in Breast Cancer. European Journal of Gynecological Oncology 2009; 30:285-8.
6. Murri AM, Hilmy M, Bell J et al. The relationship between the systemic inflammatory response, tumour proliferative activity, T lymphocytic and macrophage infiltration, microvessel density and survival in patients with primary operable breast cancer. British journal of cancer 2008; 99:1013-9.
7. Popiela TJ, Sikora J, Klimek M et al. The analysis of CD 34 antigen immunoreactivity in invasive ductal breast cancer with respect to the presence of lymph node metastasis. European journal of gynecological oncology2009;30:285-8
8. Kanjanapanjapol S, Wongwaisayawan S, Phuwapraisirisan S,Wilasrusmee C. Prognostic Significance of Microvessel density in Breast cancer of Thai women. Journal of Medical Association Thai 2007;90:282-90.
9. Uzzan B, Nicolas P, Cucherat M, Perret GY. Microvessel density as a Prognostic factor in Women with Breast Cancer: A Systematic Review of the Literature and Meta-Analysis. Cancer Res 2004; 64: 2941-2955.
10. Pansrikaew P, Cheewakriangkrai C, Taweewist M, Khunamornpong S, Siriaunkgul S. Correlation of Mast Cell Density, Angiogenesis and Clinical Outcomes in patients with Endometrioid Endometrial Cancer. Asian Pacific J Cancer Prev 2010; 11: 309-12.
11. Pyakurel D, Karki S, Agrawal CS. A study on microvascular density in breast carcinoma. Journal of Pathology of Nepal 2014; 4 :570-75.
12. Teo NB, Shoker BS, Jarvis C, Martin L, Sloane JP, Holcombe C et al. Angiogenesis and Invasive recurrence in Ductal Carcinoma in Situ of the Breast. Eur J Cancer 2003; 39:38-44.
13. Mousa SA. Mechanisms of Angiogenesis: Potential Therapeutic Targets, in Angiogenesis Inhibitors and Stimulators: Potential Therapeutic Implications . Landes Bio sci 2000: 1-12.
14. Safwat MD, Habib F, Elayat A, Oweiss N, Reffat S, Algaidi S. Morphometric and Immunohistochemical study of Angiogenic Marker expressions in Invasive Ductal Carcinomas of the Human Breast. Folia Morphol 2009;68:144-55.
15. Bolat F, Kayaselcuk F, Nursal TZ, Yagmurdur MC, Bal N, Demirhan B. Microvessel Density, VEGF Expression, and Tumor associated Macrophages in Breast Tumors: Correlations with Prognostic Parameters. J Exp Clin Cancer Res 2006;25:365-72.
16. Kwon GY, Lee SD, Park ES. Mast cell and Macrophage Counts and Microvessel Density in Invasive breast carcinoma: Comparison Analysis with Clinico Pathological Parameters. Cancer Res Treat 2005;37:103-08.
17. Amin M, Amira K, Hawary EL, Omar Farouk. Relation of CD117 immunoreactivity and microvascular density in invasive breast carcinoma. Indian Journal of Pathology and Microbiology 2012;55(4):456-60.
18. Vartanian R, Weidner N: Correlation of Intratumoral Endothelial-Cell Proliferation with Microvessel Density (Tumor Angiogenesis) and Tumor-Cell Proliferation in Breast Carcinoma. Am J Pathol 1994; 144:1188-94.
2. Kalyani R , Das S , Singh Bindra MS , Kumar HML. Cancer Profile in the Department of Pathology at Sri Devraj Urs Medical College ,Kolar : A ten years study. Indian Jr of Cancer 2010 ;47: 160-5.
3. Li HJ, Jing J, Zhao YB, Zhu JQ, Zhang SY, Shi ZD. Tumor Angiogenesis in Node Negative Breast Carcinoma. A I Zheng 2002; 21: 75-8.
4. McCarthy N J, Yang X, Linniola I R, Merino MJ, Hewitt SM, Parr AL et, al. Microvessel Density, Expression of Estrogen Receptor ,MIB 1, p53 & C erb 2 in Inflammatory Breast Cancer. Clin Cancer Res 2002; 8: 3857-62.
5. Da Silva BB, Lopes costa PV, dos Santos AR et al. Comparison of three Vascular Endothelial Markers in the Evaluation of Micro vessel Density in Breast Cancer. European Journal of Gynecological Oncology 2009; 30:285-8.
6. Murri AM, Hilmy M, Bell J et al. The relationship between the systemic inflammatory response, tumour proliferative activity, T lymphocytic and macrophage infiltration, microvessel density and survival in patients with primary operable breast cancer. British journal of cancer 2008; 99:1013-9.
7. Popiela TJ, Sikora J, Klimek M et al. The analysis of CD 34 antigen immunoreactivity in invasive ductal breast cancer with respect to the presence of lymph node metastasis. European journal of gynecological oncology2009;30:285-8
8. Kanjanapanjapol S, Wongwaisayawan S, Phuwapraisirisan S,Wilasrusmee C. Prognostic Significance of Microvessel density in Breast cancer of Thai women. Journal of Medical Association Thai 2007;90:282-90.
9. Uzzan B, Nicolas P, Cucherat M, Perret GY. Microvessel density as a Prognostic factor in Women with Breast Cancer: A Systematic Review of the Literature and Meta-Analysis. Cancer Res 2004; 64: 2941-2955.
10. Pansrikaew P, Cheewakriangkrai C, Taweewist M, Khunamornpong S, Siriaunkgul S. Correlation of Mast Cell Density, Angiogenesis and Clinical Outcomes in patients with Endometrioid Endometrial Cancer. Asian Pacific J Cancer Prev 2010; 11: 309-12.
11. Pyakurel D, Karki S, Agrawal CS. A study on microvascular density in breast carcinoma. Journal of Pathology of Nepal 2014; 4 :570-75.
12. Teo NB, Shoker BS, Jarvis C, Martin L, Sloane JP, Holcombe C et al. Angiogenesis and Invasive recurrence in Ductal Carcinoma in Situ of the Breast. Eur J Cancer 2003; 39:38-44.
13. Mousa SA. Mechanisms of Angiogenesis: Potential Therapeutic Targets, in Angiogenesis Inhibitors and Stimulators: Potential Therapeutic Implications . Landes Bio sci 2000: 1-12.
14. Safwat MD, Habib F, Elayat A, Oweiss N, Reffat S, Algaidi S. Morphometric and Immunohistochemical study of Angiogenic Marker expressions in Invasive Ductal Carcinomas of the Human Breast. Folia Morphol 2009;68:144-55.
15. Bolat F, Kayaselcuk F, Nursal TZ, Yagmurdur MC, Bal N, Demirhan B. Microvessel Density, VEGF Expression, and Tumor associated Macrophages in Breast Tumors: Correlations with Prognostic Parameters. J Exp Clin Cancer Res 2006;25:365-72.
16. Kwon GY, Lee SD, Park ES. Mast cell and Macrophage Counts and Microvessel Density in Invasive breast carcinoma: Comparison Analysis with Clinico Pathological Parameters. Cancer Res Treat 2005;37:103-08.
17. Amin M, Amira K, Hawary EL, Omar Farouk. Relation of CD117 immunoreactivity and microvascular density in invasive breast carcinoma. Indian Journal of Pathology and Microbiology 2012;55(4):456-60.
18. Vartanian R, Weidner N: Correlation of Intratumoral Endothelial-Cell Proliferation with Microvessel Density (Tumor Angiogenesis) and Tumor-Cell Proliferation in Breast Carcinoma. Am J Pathol 1994; 144:1188-94.
Published
2018-03-22
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