Seroprevalence of Transfusion transmitted infections by using 4th generation Enzyme- linked immunosorbent assay kit: A 3 year study in a tertiary health care centre of Delhi

  • Mohammad Jaseem Hassan Hamdard Institute of Medical Sciences & Research (HIMSR), Hamdard University, New Delhi
  • Sabina Khan Department of Pathology, HIMSR, Hamdard University, New Delhi.
  • Zeeba Shamim Jairajpuri Department of Pathology, HIMSR, Hamdard University, New Delhi.
  • Safia Rana Department of Pathology, HIMSR, Hamdard University, New Delhi.
  • Salamah Parveen Imteyaz Department of Physiology, SMS&R, Sharda University, Greater Noida, U.P
  • Sujata Jetley Department of Pathology, HIMSR, Hamdard University, New Delhi.
Keywords: Transfusion transmitted infections, Blood donors. HIV, Hepatitis B, Hepatitis C, Syphilis, Malaria


Background: One of the greatest challenges of the blood transfusion services is to prevent the transmission of Transfusion transmitted infections (TTI), because these unsafe blood transfusions leads to increase morbidity and mortality and eventually leads to economic burden on the society. The objective of our study was to find out the seroprevalence of TTI among blood donors at our blood bank by using 4th generation ELISA kit and to compare our study with other studies conducted at national and state level.Method: 2401 units of blood collected during 3 years period were screened for 5 infections. HIV, Hepatitis B and Hepatitis C infections were screened by using 4th generation ELISA kit. Test for syphilis was done by Rapid plasma reagin card test and test for malaria parasite was done by Advantage Mal card test.Results: A total of 2401 units were collected during the period of three years. 98.25% donors were male. 91.09% donors were replacement donors. A total of 71 blood donors (2.95%) were tested positive for any one of the TTI. Out of these 71 cases, 69 were males and only 2 were females. The overall prevalence of HIV, HBV, HCV and syphilis in our study were 0.33%, 1.7%, 0.74% and 0.16% respectively. None of our donors was tested positive for malaria.Conclusion: In order to minimize the risk of TTI, voluntary donors should be encouraged by means of educating general people about benefits of blood donation and motivating them by conducting regular blood camps.


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