Role of IL-8, IL-10 and TNF-α level in pathogenesis of leptospiral acute hepatitis syndrome

  • Meher Rizvi Assistant Professor Department of Microbiology JNMCH, AMU, Aligarh
  • Mohd Azam
  • Asfia Sultan
  • Fatima Khan
  • Indu Shukla
  • Abida Malik
  • Masihur R Ajmal
Keywords: Leptospirosis, Cytokines, ELISA, Immunopathogenesis, Hepatitis, Interleukins, TNF,


Background: Leptospirosis is the most widespread zoonosis occurring worldwide. Severity of disease may range from inapparent infection to fulminant, fatal disease. Besides, direct injury by microbial factors, cytokines produced in response to infection have been proposed to be involved in the pathogenesis of leptospirosis.Objective: This study was done to assess the impact of cytokines IL-8, IL-10 and TNF-α in pathogenesis of leptospirosis and to establish their role as diagnostic markers in disease severity.Methods: Fourty six patients with signs and symptoms of acute hepatitis who were positive for leptospira by IgM ELISA along with 30 control individuals were included in the study. Concentrations of circulating IL-8, IL-10 & TNF-α were measured in pg/ml by ELISA.Results: There were two to threefold rise in liver enzymes in patients with leptospirosis. Concentrations of IL-8, IL-10 were found to be elevated in 41 (89.1%), 26 (56.52%) patients while that of TNF-α were largely suppressed in patients with leptospirosis. Mean levels of IL-8, IL-10 & TNF-α was 175.96 pg/ml, 32.9 pg/ml and 46.49 pg/ml respectively. Higher levels of IL-8, IL-10 and lower levels of TNF-α were found to be associated with better prognosis. IL-10/TNF-α ratio was significantly high in our study.Conclusion: Ratios of IL-10/TNF-α may play a role in predicting disease prognosis as high IL-10/TNF-α ratios are associated with good prognosis. 


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Original Article